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Validation and Determination of Quinolizidine Alkaloids (QAs) in Lupin Products by Gas Chromatography with Flame Ionization Detection (GC-FID)
Analytical Letters ( IF 2 ) Pub Date : 2019-09-09 , DOI: 10.1080/00032719.2019.1661423 Hae-Won Lee 1 , In-Min Hwang 1 , Hee Min Lee 1 , Ji-Su Yang 1 , Eun Jin Park 1 , Jae Won Choi 1 , Hye Young Seo 1 , Sung Hyun Kim 1
Analytical Letters ( IF 2 ) Pub Date : 2019-09-09 , DOI: 10.1080/00032719.2019.1661423 Hae-Won Lee 1 , In-Min Hwang 1 , Hee Min Lee 1 , Ji-Su Yang 1 , Eun Jin Park 1 , Jae Won Choi 1 , Hye Young Seo 1 , Sung Hyun Kim 1
Affiliation
Abstract Products of lupin, a leguminous plant belonging to genus Lupinus, have high protein contents and are consumed in Mediterranean and Middle East countries. However, quinolizidine alkaloids (QAs) present in lupin have been reported to be toxic for humans and animals. Therefore, we carried out a quantitative determination of the QAs such as sparteine, angustifoline, lupanine, and 13-hydroxylupanine, which are known to be the major components in lupin and lupin products. The QAs were determined in three lupin products, namely, lupin beans, lupin cookies, and a lupin drink. The analysis was carried out using gas chromatography with a flame ionization detector (GC-FID) and the method was also validated. All of the QAs were eluted in 20 min, and the correlation coefficients were acceptable (R2 > 0.99). The limit of quantification in this method was lower than for a previously used capillary electrophoresis–mass spectrometric method. The total QA contents in lupin beans, lupin cookies, and lupin drink were 97.031, 4.786, and 67.887 µg/mL. Based on our results, it was concluded that these products were safe for consumption. It is believed that this method can be effectively used for the determination of QAs as GC-FID is a widely used technique.
中文翻译:
通过气相色谱-火焰离子化检测 (GC-FID) 验证和测定羽扇豆产品中的喹诺利西啶生物碱 (QA)
摘要 羽扇豆是一种属于羽扇豆属的豆科植物,蛋白质含量高,在地中海和中东国家消费。然而,据报道羽扇豆中的喹诺利西啶生物碱 (QAs) 对人类和动物有毒。因此,我们对已知是羽扇豆和羽扇豆产品中的主要成分的 QAs 进行了定量测定,例如斯巴达碱、angustifoline、羽扇豆碱和 13-羟基羽扇豆碱。在三种羽扇豆产品中确定了质量保证,即羽扇豆、羽扇豆饼干和羽扇豆饮料。使用带有火焰离子化检测器 (GC-FID) 的气相色谱法进行分析,并对该方法进行了验证。所有 QA 均在 20 分钟内洗脱,相关系数可接受(R2 > 0.99)。该方法的定量限低于以前使用的毛细管电泳-质谱法。羽扇豆、羽扇豆饼干和羽扇豆饮料中的总 QA 含量分别为 97.031、4.786 和 67.887 µg/mL。根据我们的结果,得出的结论是这些产品可以安全食用。相信该方法可以有效地用于 QA 的测定,因为 GC-FID 是一种广泛使用的技术。
更新日期:2019-09-09
中文翻译:
通过气相色谱-火焰离子化检测 (GC-FID) 验证和测定羽扇豆产品中的喹诺利西啶生物碱 (QA)
摘要 羽扇豆是一种属于羽扇豆属的豆科植物,蛋白质含量高,在地中海和中东国家消费。然而,据报道羽扇豆中的喹诺利西啶生物碱 (QAs) 对人类和动物有毒。因此,我们对已知是羽扇豆和羽扇豆产品中的主要成分的 QAs 进行了定量测定,例如斯巴达碱、angustifoline、羽扇豆碱和 13-羟基羽扇豆碱。在三种羽扇豆产品中确定了质量保证,即羽扇豆、羽扇豆饼干和羽扇豆饮料。使用带有火焰离子化检测器 (GC-FID) 的气相色谱法进行分析,并对该方法进行了验证。所有 QA 均在 20 分钟内洗脱,相关系数可接受(R2 > 0.99)。该方法的定量限低于以前使用的毛细管电泳-质谱法。羽扇豆、羽扇豆饼干和羽扇豆饮料中的总 QA 含量分别为 97.031、4.786 和 67.887 µg/mL。根据我们的结果,得出的结论是这些产品可以安全食用。相信该方法可以有效地用于 QA 的测定,因为 GC-FID 是一种广泛使用的技术。