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Aptamer-Based Lateral Flow Test Strip for the Simultaneous Detection of Salmonella typhimurium, Escherichia coli O157:H7 and Staphylococcus aureus
Analytical Letters ( IF 2 ) Pub Date : 2019-09-17 , DOI: 10.1080/00032719.2019.1663528
Chunxia Lu 1 , Xiaoxu Gao 1 , Ya Chen 2 , Jiangtao Ren 2 , Changbin Liu 3
Affiliation  

Abstract An aptamer-based lateral flow test strip was developed for the simultaneous detection of Salmonella Typhimurium, Escherichia coli O157:H7, and Staphylococcus aureus. This assay was based on the sandwich-type format using primary aptamer conjugated with gold nanoparticles (AuNPs) as the signal probe and a secondary aptamer-coated membrane as a capture probe. Several experimental conditions that influenced sensitivity were investigated, including the aptamer and NaCl concentrations and the loaded amounts of gold nanoparticle–aptamer conjugates and streptavidin–aptamer conjugates. Under the optimal experimental conditions, the visual limits of detection of the strip for S. Typhimurium, E. coli O157:H7 and S. aureus were 103 CFU/mL, 104 CFU/mL, and 104 CFU/mL, respectively. All measurements were achieved within 10 min, and no significant cross-reactivity with several foodborne pathogens was observed. This aptamer-based strip was successfully applied to the simultaneous detection of these foodborne pathogens in food samples. The aptamer-based lateral flow test strip was shown to provide a reliable, simple and accurate tool for the simultaneous detection of multiple foodborne pathogens.

中文翻译:

用于同时检测鼠伤寒沙门氏菌、大肠杆菌 O157:H7 和金黄色葡萄球菌的基于适体的侧流测试条

摘要 开发了一种基于适体的侧流检测条,用于同时检测鼠伤寒沙门氏菌、大肠杆菌O157:H7 和金黄色葡萄球菌。该测定基于夹心型格式,使用与金纳米粒子 (AuNP) 结合的初级适体作为信号探针,二级适体包被膜作为捕获探针。研究了影响灵敏度的几种实验条件,包括适体和 NaCl 浓度以及金纳米颗粒-适体偶联物和链霉亲和素-适体偶联物的负载量。在最佳实验条件下,条带对鼠伤寒沙门氏菌、大肠杆菌O157:H7 和金黄色葡萄球菌的视觉检测限分别为103 CFU/mL、104 CFU/mL 和104 CFU/mL。所有测量均在 10 分钟内完成,并且没有观察到与几种食源性病原体的显着交叉反应。这种基于适配体的条带已成功应用于食品样品中这些食源性病原体的同时检测。基于适体的横向流动测试条被证明为同时检测多种食源性病原体提供了一种可靠、简单和准确的工具。
更新日期:2019-09-17
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