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Keth-seq for transcriptome-wide RNA structure mapping
Nature Chemical Biology ( IF 14.8 ) Pub Date : 2020-02-03 , DOI: 10.1038/s41589-019-0459-3
Xiaocheng Weng 1, 2 , Jing Gong 3 , Yi Chen 2 , Tong Wu 1 , Fang Wang 1, 4 , Shixi Yang 2 , Yushu Yuan 2 , Guanzheng Luo 1 , Kai Chen 1 , Lulu Hu 1 , Honghui Ma 1 , Pingluan Wang 1 , Qiangfeng Cliff Zhang 3 , Xiang Zhou 2 , Chuan He 1
Affiliation  

RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.



中文翻译:

Keth-seq 用于转录组范围的 RNA 结构映射

RNA 二级结构对 RNA 调控和功能至关重要。我们报告了一种新的 N 3 -kethoxal 试剂,它可以快速和可逆地标记活细胞中的单链鸟嘌呤碱基。这种基于 N 3 -kethoxal 的化学方法允许在温和条件下进行有效的 RNA 标记和转录组范围的 RNA 二级结构作图。

更新日期:2020-02-03
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