Dendrobium nobile is an important medicinal food beneficial for human health, well known for polysaccharides and dendrobine. For fast, accurate, and comprehensive comparison of its quality, high performance liquid chromatography (HPLC) fingerprinting method was constructed. Firstly, spring frost stressed D. nobile herb was observed for assessment. Decreased leaf thickness, chlorophyll, and drying rate, and increased free-proline indicated heavy damages on growth. But, the content of polysaccharides increased significantly in during-frost (DF), and dropped significantly in after-frost (AF). The content of dendrobine accumulated significantly in AF. Then, low similarity among HPLC fingerprints of before-frost (BF), DF, and AF, and 75.82% of significantly variant peaks indicated the changing of much more components. Especially, some less-polar components increased significantly in DF, but not in AF. Moreover, the highest suppression rates (SRs) to A549 lung cancer cells were up to 33.08% in DF, but only 15.63% and 12.12% in BF and AF. After association analysis, eleven less-polar components were found to be significantly and positively correlated to SRs under relatively high concentration. The result shows that frost stress not only causes damages to plant growth, but also promotes the accumulation of some health-beneficial bioactive metabolites. HPLC based fingerprinting method shows good applicability on quality evaluation and bioactivity correlation analysis of complexed agricultural products.

中文翻译：

---

石no中高效液相色谱指纹图谱定量评估次生代谢产物和癌细胞的抑制活性。

end石end是对人体健康有益的重要药用食品，以多糖和石d碱闻名。为了快速，准确，全面地比较其质量，构建了高效液相色谱（HPLC）指纹图谱方法。首先，观察了春季霜冻胁迫的D. nobile药草的评估。叶片厚度，叶绿素和干燥速率的降低以及游离脯氨酸的增加表明对生长的严重损害。但是，霜冻期间（DF）的多糖含量明显增加，而霜冻后（AF）的多糖含量明显减少。石AF碱的含量在AF中明显积累。然后，霜前（BF），DF和AF的HPLC指纹图谱之间的低相似性以及显着的变异峰的75.82％表明存在更多的组分变化。特别，DF中一些极性较小的成分显着增加，而AF中则没有。此外，DF对A549肺癌细胞的最高抑制率（SRs）高达33.08％，而BF和AF仅对15.549％和12.12％。经过关联分析，发现在相对较高的浓度下，有11个极性较小的成分与SR显着正相关。结果表明，霜冻胁迫不仅对植物生长造成损害，而且还促进了一些有益健康的生物活性代谢产物的积累。基于HPLC的指纹图谱方法在复杂农产品的质量评价和生物活性相关分析中显示出良好的适用性。BF和AF中占12％。经过关联分析，发现在相对较高的浓度下，有11个极性较小的成分与SR显着正相关。结果表明，霜冻胁迫不仅对植物生长造成损害，而且还促进了一些有益健康的生物活性代谢产物的积累。基于HPLC的指纹图谱方法在复杂农产品的质量评价和生物活性相关分析中具有良好的适用性。BF和AF中占12％。经过关联分析，发现在相对较高的浓度下，有11个极性较小的成分与SR显着正相关。结果表明，霜冻胁迫不仅对植物生长造成损害，而且还促进了一些有益健康的生物活性代谢产物的积累。基于HPLC的指纹图谱方法在复杂农产品的质量评价和生物活性相关分析中显示出良好的适用性。