The efficacy of benzyl isothiocyanate (BIT), a compound found in cruciferous vegetables, for controlling Salmonella on alfalfa seeds and sprouts was investigated. Alfalfa seeds were inoculated by individual Salmonella enterica serotypes Newport, Typhimurium, Tennessee, Montevideo, or Braenderup at two inoculation levels of 3 or 5 log CFU g⁻¹, followed by treatment with BIT (0.5–2%) or chlorine (2%) for 15 min. Treated seeds were sprouted in seed sprouters. Surviving Salmonella populations on the seeds and sprouts were determined. At both inoculum levels, 1.5% and 2% of BIT significantly reduced populations S. Typhimurium and S. Braenderup on seeds to a level comparable to the chlorine‐treated seeds. Salmonella populations recovered from the sprouts germinated from 1.5% or 2% BIT‐treated seeds were significantly lower than the control and chlorine‐treated seeds at both inoculum levels (P < 0.05). The results of the study indicate that BIT can be used to control Salmonella contamination on alfalfa seeds and subsequent sprouting process.

中文翻译：

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异硫氰酸苄酯防治苜蓿种子和芽苗中沙门氏菌的功效

研究了十字花科蔬菜中发现的一种化合物异硫氰酸苄酯（BIT）防治苜蓿种子和芽上沙门氏菌的功效。苜蓿种子分别以3或5 log CFU g ^-1的两种接种水平分别接种纽波特，鼠伤寒，田纳西州，蒙得维的亚或Braenderup的单个沙门氏菌血清型，然后用BIT（0.5–2％）或氯（2％）处理15分钟 处理过的种子在种子发芽器中发芽。确定了种子和芽上存活的沙门氏菌种群。在这两个水平的接种物，1.5％和BIT的2％显著减少群体S. 鼠伤寒沙门氏菌和S. 种子上的Braenderup达到与氯处理种子相当的水平。在两种接种量下，从1.5％或2％BIT处理过的种子发芽的芽中回收的沙门氏菌种群均显着低于对照和氯处理种子（P  <0.05）。研究结果表明，BIT可用于控制苜蓿种子和后续发芽过程中沙门氏菌的污染。