A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5–11 and space-time yields of up to 22.9 g L^–1 h^–1. The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation.

中文翻译：

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碳作为连续流中氧化还原生物催化的简单载体

使用共固定在简单碳载体上的酶进行依赖于 NADH 的生物催化的连续填充床反应器被优化为在 30 分钟的停留时间内达到 100% 的转化率。丙酮酸转化为乳酸是通过共固定化乳酸脱氢酶和甲酸脱氢酶实现的，提供原位辅因子回收。其他指标也被视为优化目标，例如 2.5-11 之间的低 E 因子和高达 22.9 g L ^–1 h ^–1的时空产量。还证明了生物催化反应器的长期稳定性，在连续运行 30 多小时内保持完全转化。