BACKGROUND Targeted delivery of virus-associated antigens to professional antigen-presenting cells (APCs) is considered as an efficient strategy to enhance the pyrophytic effect of vaccines against rhabdovirus disease. MATERIALS AND METHODS In this study, we constructed a targeted carbon nanotubes-based vaccine deliver system (SWCNTs-MG) which can recognize the signature receptor (mannose) of APCs. An environmentally and economically important disease called spring viremia of carp (SVC) was studied as a model to evaluate the feasibility of single-walled carbon nanotubes (SWCNTs) conjugated with mannosylated antigen for rhabdovirus prevention. RESULTS Results showed that SWCNTs-MG could cross into fish body and present to internal immune-related tissues through gill, muscle and intestine within 6 h immersed vaccination. With further modification of mannose moiety, the obtained nanovaccine showed enhanced uptake by carp macrophages and immune-related tissues, which would then trigger strong immune responses against spring viremia of carp virus (SVCV) infection. Moreover, the survival rate of fish vaccinated with SWCNTs-MG (30 mg/L) was 63.5% after SVCV infection, whereas it was 0% for the control group. CONCLUSION This study not only provide a theoretical basis and research template for the application of targeted nanovaccine system in aquatic animals, but also play an important role in supporting development of healthy aquaculture and ensuring the safety of aquatic products and ecology.

中文翻译：

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以鱼为模型生物的抗原呈递细胞靶向的纳米疫苗递送系统在弹状病毒疾病预防中的应用。

背景技术将病毒相关抗原靶向递送至专业抗原呈递细胞（APC）被认为是增强针对弹状病毒疾病的疫苗的自热作用的有效策略。材料与方法在这项研究中，我们构建了一种靶向碳纳米管为基础的疫苗输送系统（SWCNTs-MG），该系统可以识别APC的签名受体（甘露糖）。研究了一种环境和经济上重要的疾病，称为鲤鱼春季病毒血症（SVC），作为评估单糖碳纳米管（SWCNT）结合有甘露糖基化抗原预防弹状病毒的可行性的模型。结果结果表明，SWCNTs-MG可以在鱼体中浸泡6小时之内穿越鱼体内，并通过ill，肌肉和肠道进入内部免疫相关组织。随着甘露糖部分的进一步修饰，所获得的纳米疫苗显示出鲤鱼巨噬细胞和免疫相关组织的摄取增加，然后将引发针对鲤鱼病毒（SVCV）感染的春季病毒血症的强烈免疫反应。此外，接种SVCV后，接种SWCNTs-MG（30 mg / L）的鱼的存活率为63.5％，而对照组为0％。结论本研究不仅为靶向纳米疫苗系统在水生动物中的应用提供了理论依据和研究模板，而且在支持健康水产养殖业的发展以及确保水产品和生态安全方面发挥着重要作用。然后会触发针对鲤鱼病毒（SVCV）感染的春季病毒血症的强大免疫反应。此外，接种SVCV后，接种SWCNTs-MG（30 mg / L）的鱼的存活率为63.5％，而对照组为0％。结论本研究不仅为靶向纳米疫苗系统在水生动物中的应用提供了理论依据和研究模板，而且在支持健康水产养殖业的发展以及确保水产品和生态安全方面发挥着重要作用。然后会触发针对鲤鱼病毒（SVCV）感染的春季病毒血症的强大免疫反应。此外，接种SVCV后，接种SWCNTs-MG（30 mg / L）的鱼的存活率为63.5％，而对照组为0％。结论本研究不仅为靶向纳米疫苗系统在水生动物中的应用提供了理论依据和研究模板，而且在支持健康水产养殖业的发展以及确保水产品和生态安全方面发挥着重要作用。