当前位置: X-MOL 学术Genet. Sel. Evol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genomic regions associated with muscularity in beef cattle differ in five contrasting cattle breeds.
Genetics Selection Evolution ( IF 4.1 ) Pub Date : 2020-01-30 , DOI: 10.1186/s12711-020-0523-1
Jennifer L Doyle 1, 2 , Donagh P Berry 1 , Roel F Veerkamp 3 , Tara R Carthy 1 , Ross D Evans 4 , Siobhán W Walsh 2 , Deirdre C Purfield 1
Affiliation  

BACKGROUND Linear type traits, which reflect the muscular characteristics of an animal, could provide insight into how, in some cases, morphologically very different animals can yield the same carcass weight. Such variability may contribute to differences in the overall value of the carcass since primal cuts vary greatly in price; such variability may also hinder successful genome-based association studies. Therefore, the objective of our study was to identify genomic regions that are associated with five muscularity linear type traits and to determine if these significant regions are common across five different breeds. Analyses were carried out using linear mixed models on imputed whole-genome sequence data in each of the five breeds, separately. Then, the results of the within-breed analyses were used to conduct an across-breed meta-analysis per trait. RESULTS We identified many quantitative trait loci (QTL) that are located across the whole genome and associated with each trait in each breed. The only commonality among the breeds and traits was a large-effect pleiotropic QTL on BTA2 that contained the MSTN gene, which was associated with all traits in the Charolais and Limousin breeds. Other plausible candidate genes were identified for muscularity traits including PDE1A, PPP1R1C and multiple collagen and HOXD genes. In addition, associated (gene ontology) GO terms and KEGG pathways tended to differ between breeds and between traits especially in the numerically smaller populations of Angus, Hereford, and Simmental breeds. Most of the SNPs that were associated with any of the traits were intergenic or intronic SNPs located within regulatory regions of the genome. CONCLUSIONS The commonality between the Charolais and Limousin breeds indicates that the genetic architecture of the muscularity traits may be similar in these breeds due to their similar origins. Conversely, there were vast differences in the QTL associated with muscularity in Angus, Hereford, and Simmental. Knowledge of these differences in genetic architecture between breeds is useful to develop accurate genomic prediction equations that can operate effectively across breeds. Overall, the associated QTL differed according to trait, which suggests that breeding for a morphologically different (e.g. longer and wider versus shorter and smaller) more efficient animal may become possible in the future.

中文翻译:

与肉牛肌肉相关的基因组区域在五个不同的牛品种中有所不同。

背景技术反映动物的肌肉特征的线性型特征可以提供洞察力,在某些情况下,形态上非常不同的动物如何产生相同的car体重量。由于原始割价的差异很大,因此这种可变性可能会导致s体总价值的差异。这种可变性也可能阻碍成功的基于基因组的关联研究。因此,我们研究的目的是确定与五个肌肉线性类型性状相关的基因组区域,并确定这些重要区域在五个不同品种中是否相同。使用线性混合模型分别对五个品种中的估算全基因组序列数据进行了分析。然后,品种内分析的结果用于对每个性状进行品种间荟萃分析。结果我们确定了许多数量性状基因座(QTL),它们位于整个基因组中,并与每个品种的每个性状相关。品种和性状之间唯一的共同点是对BTA2的大效多效性QTL,其中包含MSTN基因,该基因与夏洛来和利穆赞种的所有性状有关。确定了其他可能的肌肉性状候选基因,包括PDE1A,PPP1R1C和多种胶原和HOXD基因。此外,相关的(基因本体论)GO术语和KEGG途径在品种之间和性状之间往往有所不同,尤其是在数量较少的安格斯,赫里福德和西门塔尔品种中。与任何性状相关的大多数SNP是位于基因组调控区内的基因间或内含子SNP。结论夏洛来和利穆赞品种之间的共性表明,由于其相似的起源,这些品种的肌肉性状的遗传结构可能相似。相反,在安格斯,赫里福德和西门塔尔,与肌肉相关的QTL存在巨大差异。了解不同品种之间遗传结构的这些差异对于开发可以在不同品种之间有效运行的准确基因组预测方程非常有用。总体而言,相关的QTL根据性状而有所不同,这表明将来可能会在形态上不同(例如更长和更宽而更短和更小)的动物中进行育种。
更新日期:2020-04-22
down
wechat
bug