BACKGROUND Platelets undergo structural, biochemical and functional alterations when stored, and platelet storage lesions reduce platelet function and half-life after transfusion. The objective of this study was to evaluate stored canine platelet concentrates with platelet aggregation, flow cytometry and biochemistry assays. Twenty-two bags of canine platelet concentrates were obtained by the platelet-rich plasma method and were assessed on days 1, 3 and 5 after collection. Parameters such as platelet counts, residual leukocytes, platelet swirling, glucose, lactate, pH, CD62P expression (platelet activation), JC-1 (mitochondrial function) and annexin V (apoptosis and cell death) were assessed. RESULTS Over the five days of storage there was a significant decrease in glucose, HCO3, pCO2, ATP, pH, swirling and mitochondrial function, associated with a significant increase in lactate levels and pO2. At the end of storage pH was 5.9 ± 0.6 and lactate levels were 2.8 ± 1.2 mmol/L. Results of the quality parameters evaluated were similar to those reported in human platelets studies. The deleterious effects of storage were more pronounced in bags with higher platelet counts (> 7.49 × 1010/unit), suggesting that canine platelet concentrates should not contain an excessive number of platelets. CONCLUSIONS Quality parameters of canine platelets under standard storage conditions were similar to those observed in human platelets. Our results have potential to be used for the routine evaluation and quality control in veterinary blood banks.

中文翻译：

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兽医血库的质量控制：评估保存五天的犬血小板浓缩液。

背景技术血小板在储存时会发生结构，生化和功能的改变，并且血小板储存损伤会降低输血后的血小板功能和半衰期。这项研究的目的是通过血小板聚集，流式细胞术和生物化学分析评估犬的血小板浓缩液。通过富含血小板的血浆方法获得22袋犬血小板浓缩物，并在收集后的第1、3和5天进行评估。评估参数，如血小板计数，残留白细胞，血小板漩涡，葡萄糖，乳酸，pH，CD62P表达（血小板活化），JC-1（线粒体功能）和膜联蛋白V（凋亡和细胞死亡）。结果在储存的五天中，葡萄糖，HCO3，pCO2，ATP，pH，涡旋和线粒体功能显着下降，与乳酸水平和pO2的显着增加有关。储存结束时，pH为5.9±0.6，乳酸水平为2.8±1.2 mmol / L。评估的质量参数结果与人体血小板研究报告的结果相似。在具有更高血小板计数（> 7.49×1010 /单位）的袋子中，储藏的有害影响更为明显，这表明犬浓缩血小板中不应含有过多的血小板。结论标准储存条件下犬血小板的质量参数与人血小板中观察到的参数相似。我们的结果有潜力用于兽医血库的常规评估和质量控制。评估的质量参数结果与人体血小板研究报告的结果相似。在具有更高血小板计数（> 7.49×1010 /单位）的袋子中，储存的有害影响更为明显，这表明犬浓缩血小板中不应含有过多的血小板。结论标准储存条件下犬血小板的质量参数与人血小板中观察到的参数相似。我们的结果有潜力用于兽医血库的常规评估和质量控制。评估的质量参数结果与人体血小板研究报告的结果相似。在具有更高血小板计数（> 7.49×1010 /单位）的袋子中，储藏的有害影响更为明显，这表明犬浓缩血小板中不应含有过多的血小板。结论标准储存条件下犬血小板的质量参数与人血小板中观察到的参数相似。我们的结果有潜力用于兽医血库的常规评估和质量控制。提示犬血小板浓缩液不应含有过多的血小板。结论标准储存条件下犬血小板的质量参数与人血小板中观察到的参数相似。我们的结果有潜力用于兽医血库的常规评估和质量控制。提示犬血小板浓缩液不应含有过多的血小板。结论标准储存条件下犬血小板的质量参数与人血小板中观察到的参数相似。我们的结果有潜力用于兽医血库的常规评估和质量控制。