BACKGROUND Fusarium wilt of banana is one of the most destructive diseases in banana-growing regions worldwide. Soil-borne diseases and soil microbial communities are closely related. The screening of antagonistic bacteria from soil microorganisms in areas with Fusarium wilt of banana is of great practical significance for controlling this disease. RESULTS A strain designated FS-4 was isolated from healthy banana rhizosphere soil in an area affected by Fusarium wilt. This strain exhibited a significant antagonistic effect on the pathogen. Pot experiments revealed that the fermentation broth of strain FS-4 not only decreased the incidence of banana Fusarium wilt, but also promoted the growth of banana seedlings. The strain was identified as Streptomyces ma. by its morphological, physiological, and biochemical characteristics and 16S rRNA gene sequence analysis. The culture and fermentation conditions for this strain were optimized by single-factor and response surface experiments. The optimum culture conditions for Streptomyces ma. FS-4 were as follows: peptone 0.5%, saccharose 2.4, 0.05% K2HPO4, 0.05% MgCl2, and 0.05% NaCl at an initial pH of 7.0; 180 g at 28 °C; and inoculation size of 6% for 62 h. The diameter of bacteriostasis circle for Bacillus subtilis reached 26.7 mm. CONCLUSION Streptomyces ma. FS-4 is an important microbial resource as a biological agent for the control of plant pathogenic fungi and can be used to promote banana growth.

中文翻译：

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链霉菌的发酵优化和抑病能力。香蕉根际土壤中的FS-4。

背景技术香蕉枯萎病是全世界香蕉种植地区最具破坏性的疾病之一。土壤传播疾病与土壤微生物群落密切相关。从香蕉枯萎病地区的土壤微生物中筛选拮抗细菌对控制该病具有重要的现实意义。结果从健康的香蕉根际土壤中分离到一个名称为FS-4的菌株，该土壤受镰刀菌枯萎病的影响。该菌株对病原体表现出明显的拮抗作用。盆栽试验表明，FS-4菌株的发酵液不仅降低了香蕉枯萎病的发生率，而且促进了香蕉幼苗的生长。该菌株被鉴定为马链霉菌。根据其形态，生理，的生化特性和16S rRNA基因序列分析。通过单因素和响应面实验优化了该菌株的培养和发酵条件。链霉菌的最佳培养条件。FS-4如下：蛋白pH0.5％，蔗糖2.4、0.05％K 2 HPO 4，0.05％MgCl 2和0.05％NaCl，初始pH为7.0。在28°C下为180克；接种量为6％，持续62小时。枯草芽孢杆菌的抑菌圈直径达到26.7 mm。结论链霉菌 FS-4是一种重要的微生物资源，可作为控制植物病原性真菌的生物制剂，可用于促进香蕉的生长。FS-4如下：蛋白pH0.5％，蔗糖2.4、0.05％K 2 HPO 4，0.05％MgCl 2和0.05％NaCl，初始pH为7.0。在28°C下为180克；接种量为6％，持续62小时。枯草芽孢杆菌的抑菌圈直径达到26.7 mm。结论链霉菌 FS-4是一种重要的微生物资源，可作为控制植物病原性真菌的生物制剂，可用于促进香蕉的生长。FS-4如下：蛋白pH0.5％，蔗糖2.4、0.05％K 2 HPO 4，0.05％MgCl 2和0.05％NaCl，初始pH为7.0。在28°C下为180克；接种量为6％，持续62小时。枯草芽孢杆菌的抑菌圈直径达到26.7 mm。结论链霉菌 FS-4是一种重要的微生物资源，可作为控制植物病原性真菌的生物制剂，可用于促进香蕉的生长。