Abstract Optimal production of lignocellulosic bioethanol is hindered due to commonly faced issues with the presence of inhibitory compounds and sequentially consumed sugars in the lignocellulosic hydrolysate. Therefore, in order to find a robust fermentation approach, this study aimed at enhancing simultaneous co-assimilation of sugars, and inhibitor tolerance and detoxification. Therefore, fermentation of toxic wheat straw hydrolysate containing up to 20 g/l furfural, using the concentration-driven diffusion-based technique of reverse membrane bioreactor (rMBR) was studied. The rMBR fermentation of the hydrolysate led to complete furfural detoxification and the conversion of 87 % of sugars into ethanol at a yield of 0.48 g/g. Moreover, when the toxicity level of the hydrolysate was increased to 9 g/l of initial furfural, the system responded exceptionally by reducing 89 % of the inhibitor while only experiencing about 25 % drop in the ethanol yield. In addition, using this diffusion-based set-up in extremely inhibitory conditions (16 g/l furfural), cells could detoxify 40 % of the furfural at a high initial furfural to cell ratio of 9.5:1. The rMBR set-up applied proved that by properly synchronizing the medium condition, membrane area, and inhibitor to cell ratio, some of the shortcomings with conventional lignocellulosic fermentation can be tackled, guaranteeing a robust fermentation.

中文翻译：

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用于发酵高抑制性木质纤维素水解物的浓度驱动反膜生物反应器

摘要 木质纤维素生物乙醇的最佳生产受到阻碍，这是由于木质纤维素水解物中存在抑制性化合物和连续消耗糖类的常见问题。因此，为了找到一种稳健的发酵方法，本研究旨在增强糖的同时共同化、抑制剂耐受性和解毒能力。因此，使用基于浓度驱动扩散的逆向膜生物反应器 (rMBR) 技术对含有高达 20 g/l 糠醛的有毒小麦秸秆水解物进行发酵进行了研究。水解产物的 rMBR 发酵导致糠醛完全解毒，87% 的糖以 0.48 g/g 的产量转化为乙醇。此外，当水解产物的毒性水平增加到 9 g/l 初始糠醛时，该系统通过减少 89% 的抑制剂而做出异常反应，而乙醇产量仅下降约 25%。此外，在极其抑制的条件下（16 g / l 糠醛）使用这种基于扩散的设置，细胞可以在 9.5:1 的高初始糠醛与细胞比下解毒 40% 的糠醛。应用的 rMBR 设置证明，通过适当地同步培养基条件、膜面积和抑制剂与细胞比，可以解决传统木质纤维素发酵的一些缺点，保证发酵的稳健性。在 9.5:1 的高初始糠醛与细胞比下，细胞可以解毒 40% 的糠醛。应用的 rMBR 设置证明，通过适当地同步培养基条件、膜面积和抑制剂与细胞比，可以解决传统木质纤维素发酵的一些缺点，保证发酵的稳健性。在 9.5:1 的高初始糠醛与细胞比下，细胞可以解毒 40% 的糠醛。应用的 rMBR 设置证明，通过适当地同步培养基条件、膜面积和抑制剂与细胞比，可以解决传统木质纤维素发酵的一些缺点，保证发酵的稳健性。