Abstract TCP transcription factors function as significant regulators in plant development and stress defense. In this study, a TCP transcription factor, TCP10, was isolated from moso bamboo (Phyllostachys edulis), and its expression level was induced under drought and abscisic acid (ABA)-treatment conditions. A distinct role in regulating drought-stress responses was identified. PeTCP10 conferred drought tolerance of transgenic Arabidopsis and rice as determined through phenotypic and stress-associated physiological indicators analysis, including relative water content, root growth, survival rate, germination rate, as well as malondialdehyde content. Additionally, ABA sensitivity decreased in transgenic Arabidopsis and rice compared with wild-type plants; over-expressing PeTCP10 in Arabidopsis promoted stomatal closure under ABA-treatment conditions, and ABA accumulated was non-significant difference between transgenic and wild-type Arabidopsis under drought condition. PeTCP10 inhibited lateral root growth via Me-JA (Methyl jasmonate) dependent pathway, while increasing taproot growth under continuous mannitol treatments during the seedling stage in transgenic Arabidopsis. RNA-sequencing revealed that PeTCP10 regulated the expression of a wide spectrum of stress-responsive DEGs. Yeast one hybrid and EMSA assays showed that stress-/ABA-responsive gene BT2 was the directly target gene of PeTCP10. Thus, PeTCP10 may have positive regulatory functions in the drought tolerance of plants through an ABA-dependent signaling pathway and negative in lateral root growth via Me-JA mediated pathway.

中文翻译：

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TCP10，毛竹（Phyllostachys edulis）中的一种TCP转录因子，赋予转基因植物耐旱性

摘要 TCP 转录因子在植物发育和逆境防御中起重要调节作用。在这项研究中，TCP 转录因子 TCP10 从毛竹（Phyllostachys edulis）中分离出来，并在干旱和脱落酸 (ABA) 处理条件下诱导其表达水平。确定了在调节干旱胁迫反应中的独特作用。PeTCP10 赋予转基因拟南芥和水稻耐旱性，这是通过表型和胁迫相关生理指标分析确定的，包括相对含水量、根生长、存活率、发芽率以及丙二醛含量。此外，与野生型植物相比，转基因拟南芥和水稻的 ABA 敏感性降低；拟南芥中过表达PeTCP10促进ABA处理条件下气孔关闭，干旱条件下转基因拟南芥与野生型拟南芥的ABA积累差异不显着。PeTCP10 通过 Me-JA（茉莉酸甲酯）依赖性途径抑制侧根生长，同时在转基因拟南芥幼苗阶段在连续甘露醇处理下增加主根生长。RNA 测序显示 PeTCP10 可调节多种应激反应性 DEG 的表达。酵母一杂交和 EMSA 分析表明，应激/ABA 响应基因 BT2 是 PeTCP10 的直接靶基因。因此，PeTCP10 可能通过 ABA 依赖性信号通路在植物的耐旱性中具有正调节功能，并通过 Me-JA 介导的通路在侧根生长中具有负调节功能。干旱条件下转基因拟南芥与野生型拟南芥的ABA积累量无显着差异。PeTCP10 通过 Me-JA（茉莉酸甲酯）依赖性途径抑制侧根生长，同时在转基因拟南芥幼苗阶段在连续甘露醇处理下增加主根生长。RNA 测序显示 PeTCP10 可调节多种应激反应性 DEG 的表达。酵母一杂交和 EMSA 分析表明，应激/ABA 响应基因 BT2 是 PeTCP10 的直接靶基因。因此，PeTCP10 可能通过 ABA 依赖性信号通路在植物的耐旱性中具有正调节功能，并通过 Me-JA 介导的通路在侧根生长中具有负调节功能。干旱条件下转基因拟南芥与野生型拟南芥的ABA积累量无显着差异。PeTCP10 通过 Me-JA（茉莉酸甲酯）依赖性途径抑制侧根生长，同时在转基因拟南芥幼苗阶段在连续甘露醇处理下增加主根生长。RNA 测序显示 PeTCP10 可调节多种应激反应性 DEG 的表达。酵母一杂交和 EMSA 分析表明，应激/ABA 响应基因 BT2 是 PeTCP10 的直接靶基因。因此，PeTCP10 可能通过 ABA 依赖性信号通路在植物的耐旱性中具有正调节功能，并通过 Me-JA 介导的通路在侧根生长中具有负调节功能。