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5Gs for crop genetic improvement.
Current Opinion in Plant Biology ( IF 9.5 ) Pub Date : 2020-01-28 , DOI: 10.1016/j.pbi.2019.12.004
Rajeev K Varshney 1 , Pallavi Sinha 1 , Vikas K Singh 2 , Arvind Kumar 3 , Qifa Zhang 4 , Jeffrey L Bennetzen 5
Affiliation  

Here we propose a 5G breeding approach for bringing much-needed disruptive changes to crop improvement. These 5Gs are Genome assembly, Germplasm characterization, Gene function identification, Genomic breeding (GB), and Gene editing (GE). In our view, it is important to have genome assemblies available for each crop and a deep collection of germplasm characterized at sequencing and agronomic levels for identification of marker-trait associations and superior haplotypes. Systems biology and sequencing-based mapping approaches can be used to identify genes involved in pathways leading to the expression of a trait, thereby providing diagnostic markers for target traits. These genes, markers, haplotypes, and genome-wide sequencing data may be utilized in GB and GE methodologies in combination with a rapid cycle breeding strategy.

中文翻译:

5G用于作物遗传改良。

在这里,我们提出了一种5G育种方法,以便为作物改良带来急需的破坏性变化。这些5G是基因组装配,种质鉴定,基因功能鉴定,基因组育种(GB)和基因编辑(GE)。我们认为,重要的是要为每种作物提供基因组装配,并在测序和农艺学水平上表征深度的种质资源,以鉴定标记-性状关联和优良的单倍型。系统生物学和基于测序的作图方法可用于鉴定参与导致性状表达的途径的基因,从而为目标性状提供诊断标记。这些基因,标记,单倍型和全基因组测序数据可以结合快速循环育种策略用于GB和GE方法学中。
更新日期:2020-01-28
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