当前位置: X-MOL 学术Plant Cell Environ. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
VIPP2 interacts with VIPP1 and HSP22E/F at chloroplast membranes and modulates a retrograde signal for HSP22E/F gene expression.
Plant, Cell & Environment ( IF 7.3 ) Pub Date : 2020-02-28 , DOI: 10.1111/pce.13732
Jasmine Theis 1 , Justus Niemeyer 1 , Stefan Schmollinger 1 , Fabian Ries 2 , Mark Rütgers 1 , Tilak Kumar Gupta 3 , Frederik Sommer 1 , Ligia Segatto Muranaka 1 , Benedikt Venn 1 , Miriam Schulz-Raffelt 1 , Felix Willmund 2 , Benjamin D Engel 3 , Michael Schroda 1
Affiliation  

VIPP proteins aid thylakoid biogenesis and membrane maintenance in cyanobacteria, algae, and plants. Some members of the Chlorophyceae contain two VIPP paralogs termed VIPP1 and VIPP2, which originate from an early gene duplication event during the evolution of green algae. VIPP2 is barely expressed under nonstress conditions but accumulates in cells exposed to high light intensities or H2 O2 , during recovery from heat stress, and in mutants with defective integration (alb3.1) or translocation (secA) of thylakoid membrane proteins. Recombinant VIPP2 forms rod-like structures in vitro and shows a strong affinity for phosphatidylinositol phosphate. Under stress conditions, >70% of VIPP2 is present in membrane fractions and localizes to chloroplast membranes. A vipp2 knock-out mutant displays no growth phenotypes and no defects in the biogenesis or repair of photosystem II. However, after exposure to high light intensities, the vipp2 mutant accumulates less HSP22E/F and more LHCSR3 protein and transcript. This suggests that VIPP2 modulates a retrograde signal for the expression of nuclear genes HSP22E/F and LHCSR3. Immunoprecipitation of VIPP2 from solubilized cells and membrane-enriched fractions revealed major interactions with VIPP1 and minor interactions with HSP22E/F. Our data support a distinct role of VIPP2 in sensing and coping with chloroplast membrane stress.

中文翻译:

VIPP2在叶绿体膜上与VIPP1和HSP22E / F相互作用,并调节HSP22E / F基因表达的逆行信号。

VIPP蛋白有助于类蓝藻,藻类和植物中类囊体的生物发生和膜维持。绿藻科的某些成员包含两个VIPP同源物,分别称为VIPP1和VIPP2,它们源于绿藻进化过程中的早期基因复制事件。VIPP2在非胁迫条件下几乎不表达,但会在暴露于高光强度或H2 O2的细胞中,从热胁迫中恢复期间以及在类囊体膜蛋白的整合(alb3.1)或易位(secA)突变的突变体中积累。重组VIPP2在体外形成杆状结构,并显示出对磷脂酰肌醇磷酸的强亲和力。在胁迫条件下,> 70%的VIPP2存在于膜级分中,并位于叶绿体膜上。vipp2基因敲除突变体在光生系统II的生物发生或修复中不显示生长表型,也没有缺陷。但是,暴露于高光强度后,vipp2突变体积累的HSP22E / F更少,而LHCSR3的蛋白和转录物更多。这表明VIPP2调节用于表达核基因HSP22E / F和LHCSR3的逆行信号。从溶解的细胞和膜富集的部分中免疫沉淀VIPP2揭示了与VIPP1的主要相互作用和与HSP22E / F的次要相互作用。我们的数据支持VIPP2在感知和应对叶绿体膜应力中的独特作用。这表明VIPP2调节用于表达核基因HSP22E / F和LHCSR3的逆行信号。从溶解的细胞和膜富集的部分中免疫沉淀VIPP2揭示了与VIPP1的主要相互作用和与HSP22E / F的次要相互作用。我们的数据支持VIPP2在感知和应对叶绿体膜应力中的独特作用。这表明VIPP2调节用于表达核基因HSP22E / F和LHCSR3的逆行信号。从溶解的细胞和膜富集的部分中免疫沉淀VIPP2揭示了与VIPP1的主要相互作用和与HSP22E / F的次要相互作用。我们的数据支持VIPP2在感知和应对叶绿体膜应力中的独特作用。
更新日期:2020-02-28
down
wechat
bug