Light-regulated modules offer unprecedented new ways to control cellular behaviour with precise spatial and temporal resolution. Among a variety of bacterial light-switchable gene expression systems, single-component systems consisting of single transcription factors would be more useful due to the advantages of speed, simplicity, and versatility. In the present study, we developed a single-component light-activated bacterial gene expression system (eLightOn) based on a novel LOV domain from Rhodobacter sphaeroides (RsLOV). The eLightOn system showed significant improvements over the existing single-component bacterial light-activated expression systems, with benefits including a high ON/OFF ratio of >500-fold, a high activation level, fast activation kinetics, and/or good adaptability. Additionally, the induction characteristics, including regulatory windows, activation kinetics and light sensitivities, were highly tunable by altering the expression level of LexRO. We demonstrated the usefulness of the eLightOn system in regulating cell division and swimming by controlling the expression of the FtsZ and CheZ genes, respectively, as well as constructing synthetic Boolean logic gates using light and arabinose as the two inputs. Taken together, our data indicate that the eLightOn system is a robust and highly tunable tool for quantitative and spatiotemporal control of bacterial gene expression.

中文翻译：

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单组分光传感器系统允许高度可调和直接激活细菌细胞中的基因表达。

光调节模块提供了前所未有的新方法来以精确的空间和时间分辨率控制细胞行为。在多种细菌可光切换基因表达系统中，由于速度，简单性和多功能性的优势，由单转录因子组成的单组分系统将更加有用。在本研究中，我们基于球形球形红细菌（RsLOV）的新型LOV域开发了单组分光激活细菌基因表达系统（eLightOn）。与现有的单组分细菌光激活表达系统相比，eLightOn系统显示出显着改进，其优势包括> 500倍的高ON / OFF比，高激活水平，快速激活动力学和/或良好的适应性。另外，感应特性 包括调节窗口，激活动力学和光敏性，都可以通过改变LexRO的表达水平进行高度调节。我们分别通过控制FtsZ和CheZ基因的表达，以及使用光和阿拉伯糖作为两个输入构建合成布尔逻辑门，证明了eLightOn系统在调节细胞分裂和游泳中的有用性。综上所述，我们的数据表明eLightOn系统是用于定量和时空控制细菌基因表达的强大且高度可调的工具。以及使用光和阿拉伯糖作为两个输入来构建合成布尔逻辑门。综上所述，我们的数据表明eLightOn系统是用于定量和时空控制细菌基因表达的强大且高度可调的工具。以及使用光和阿拉伯糖作为两个输入来构建合成布尔逻辑门。综上所述，我们的数据表明eLightOn系统是用于定量和时空控制细菌基因表达的强大且高度可调的工具。