Optimal serum protein concentrations are vital for normal body functioning. Affordable while accurate protein quantification methods with minimum processing requirements are needed for diagnosis of related diseases. The standard automated chemistry analyzer is limited by high installation and maintenance costs. This study proposes the use of electrical impedimetric spectroscopy (EIS) as an alternative to current methods. Its practical applicability was tested using albumin and γ‐globulin or their miscellanea in three different media; water, serum and tissue‐mimicking phantoms at 25 °C. Impedance measurements were taken between frequency f=0.10 MHz to 300 MHz by an impedance analyzer. A Cole‐Cole analysis was used to elucidate the stepwise variations in the dielectric parameters of the protein medium so as to obtain empirical dielectric parameter‐protein concentration relationships and their correlation coefficients R². From the results, linear relationships between parameters and protein concentrations with high correlation coefficients over R²=0.90 were observed. Resistance to charge transfer R_ct and characteristic frequency f_c were significantly altered by changing protein concentrations as compared to bulk solution resistance R_s, relaxation time constant τ and shape factor α. The relationships developed would aid in monitoring changes in body fluid protein concentrations by EIS.

中文翻译：

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通过电阻抗谱对白蛋白和γ-球蛋白的Cole-Cole介电弛豫分析进行蛋白质定量

最佳血清蛋白浓度对于正常的身体功能至关重要。诊断相关疾病需要价格合理且准确的蛋白质定量方法，且处理要求最低。标准的自动化学分析仪受安装和维护成本高的限制。这项研究建议使用电阻抗谱（EIS）作为当前方法的替代方法。在三种不同的介质中使用白蛋白和γ-球蛋白或它们的混杂物测试了其实际适用性。水，血清和模仿组织的幻像，温度为25°C。在频率f之间进行阻抗测量阻抗分析仪在= 0.10 MHz至300 MHz范围内。使用Cole-Cole分析来阐明蛋白质介质介电参数的逐步变化，以获得经验的介电参数-蛋白质浓度关系及其相关系数R ²。从结果中，观察到参数与蛋白质浓度之间的线性关系，在R ² = 0.90上具有高相关系数。与本体溶液电阻R _s，弛豫时间常数τ相比，通过改变蛋白质浓度，电荷转移电阻R _ct和特征频率f _c显着改变。和形状因子α。建立的关系将有助于通过EIS监测体液中蛋白质浓度的变化。