Graptopetalum paraguayense E. Walther is an edible succulent plant that is native to Mexico. When leaves of G. paraguayense are detached from the mother plant, shoots and roots regenerate from the leaf base. To investigate the endogenous hormonal regulation of plant regeneration in G. paraguayense, we evaluated the effects of applying gibberellin (GA), abscisic acid (ABA), and phytohormone biosynthesis inhibitors to leaves detached from the mother plant. GA3 and paclobutrazol did not affect plant regeneration of G. paraguayense, whereas ABA treatment significantly suppressed plant regeneration. When leaves were treated with the ABA biosynthesis inhibitor fluridone at 10 μM, the frequency of shoot regeneration was more than twice that of the control on the 6th day after leaf detachment. In addition, when both fluridone and ABA were applied at the same time, the frequency of plant regeneration decreased to control levels or below. Endogenous ABA in leaves was approximately 1.3 ng gFW−1 just after leaf detachment, decreased to 0.3 ng gFW−1 by the 1st day after leaf detachment, and increased to 3.0 ng gFW−1 by the 9th day after leaf detachment. These findings indicate that ABA plays an important role in suppression of plant regeneration in G. paraguayense. Although plant regeneration during storage and transportation currently reduces the economic value of G. paraguayense, our findings may provide a method to delay unwanted plant formation at the leaf base.

中文翻译：

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赤霉素和脱落酸对Graptopetalum paraguayense叶片无性繁殖的影响

Graptopetalum paraguayense E. Walther 是一种原产于墨西哥的可食用多肉植物。当 G. paraguayense 的叶子与母株分离时，芽和根从叶基部再生。为了研究 G. paraguayense 植物再生的内源性激素调节，我们评估了将赤霉素 (GA)、脱落酸 (ABA) 和植物激素生物合成抑制剂应用于与母株分离的叶子的效果。GA3 和多效唑不影响 G. paraguayense 的植物再生，而 ABA 处理显着抑制植物再生。当叶子用 10 μM 的 ABA 生物合成抑制剂氟啶酮处理时，在叶子脱离后第 6 天，芽再生的频率是对照的两倍多。此外，当氟啶酮和ABA同时施用时，植物再生频率降低至对照水平或以下。叶片脱落后，叶片中的内源 ABA 约为 1.3 ng gFW-1，脱落后第 1 天降至 0.3 ng gFW-1，并在脱落后第 9 天增加至 3.0 ng gFW-1。这些发现表明 ABA 在抑制 G. paraguayense 植物再生中起重要作用。尽管目前在储存和运输过程中植物再生会降低 G. paraguayense 的经济价值，但我们的研究结果可能提供一种方法来延迟叶基部不需要的植物形成。叶片脱落后第 1 天为 3 ng gFW-1，叶片脱落后第 9 天增加到 3.0 ng gFW-1。这些发现表明 ABA 在抑制 G. paraguayense 植物再生中起重要作用。尽管目前在储存和运输过程中植物再生会降低 G. paraguayense 的经济价值，但我们的研究结果可能提供一种方法来延迟叶基部不需要的植物形成。叶片脱落后第 1 天为 3 ng gFW-1，叶片脱落后第 9 天增加至 3.0 ng gFW-1。这些发现表明 ABA 在抑制 G. paraguayense 植物再生中起重要作用。尽管目前在储存和运输过程中植物再生会降低 G. paraguayense 的经济价值，但我们的研究结果可能提供一种方法来延迟叶基部不需要的植物形成。