Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-related proteins. High-throughput transcriptome investigations of plant immunity highlight the complexity of gene networks leading to incompatible interaction with the pathogen. Accumulating findings implicate papain-like cysteine proteases (PLCPs) as a central hub in plant defense. While diverse roles of PLCPs in different pathosystems have become more evident, information on gene networks and signaling pathways necessary to orchestrate downstream responses are lacking. To understand the biological significance of cysteine protease against Xanthomonas oryzae pv. oryzae, PLCP overexpression and knockout rice lines were generated. The pathogenicity test revealed the attenuation of Xanthomonas oryzae pv. oryzae race K3a virulence in transgenic lines which is ascribed to high hydrogen peroxide and free salicylic acid accumulation. Next-generation sequencing of RNA from transgenic and wild-type plants identified 1597 combined differentially expressed genes, 1269 of which were exclusively regulated in the transgenic libraries. It was found that PLCP aids rice to circumvent infection through the extensive activation of transduction signal and transcription factors that orchestrate downstream responses, including up-regulation of multiple pathogenesis-related proteins and biosynthesis of secondary metabolites.

中文翻译：

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木瓜蛋白酶样半胱氨酸蛋白酶介导的对米黄单胞菌的抗性的全基因组转录反应 pv。稻米中的稻米。

过表达 PLCP 的转基因水稻减弱了 Xanthomonas oryzae pv. 的毒力。通过广泛激活转导信号和转录活动，协调下游反应，包括次生代谢物的生物合成和几种发病机制相关蛋白的上调，稻瘟病菌。植物免疫的高通量转录组研究突出了导致与病原体相互作用不相容的基因网络的复杂性。越来越多的发现暗示木瓜蛋白酶样半胱氨酸蛋白酶 (PLCPs) 是植物防御的中心枢纽。虽然 PLCP 在不同病理系统中的不同作用变得更加明显，但缺乏有关协调下游反应所需的基因网络和信号通路的信息。了解半胱氨酸蛋白酶对抗米黄单胞菌的生物学意义。产生了 oryzae、PLCP 过表达和敲除水稻品系。致病性试验显示米黄单胞菌 pv 的减毒。转基因株系中稻瘟病菌小种 K3a 的毒力归因于高过氧化氢和游离水杨酸的积累。来自转基因和野生型植物的 RNA 的下一代测序确定了 1597 个组合的差异表达基因，其中 1269 个在转基因文库中被专门调控。研究发现，PLCP 通过广泛激活调节下游反应的转导信号和转录因子来帮助水稻规避感染，包括多种发病机制相关蛋白的上调和次生代谢物的生物合成。