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Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea.
Plant Cell Reports ( IF 6.2 ) Pub Date : 2020-01-28 , DOI: 10.1007/s00299-020-02508-0
José M Alvarez 1 , Natalia Bueno 1 , Candela Cuesta 1 , Isabel Feito 2 , Ricardo J Ordás 1
Affiliation  

Several members of WOX and KNOX gene families and several plant growth regulators, basically cytokinins and auxins, play a key role during adventitious caulogenesis in the conifer Pinus pinea. Similar to Arabidopsis thaliana, Pinus pinea shoot organogenesis is a multistep process. However, there are key differences between both species, which may alter the underlying physiological and genetic programs. It is unknown if the genic expression models during angiosperm development may be applicable to conifers. In this work, an analysis of the endogenous content of different plant growth regulators and the expression of genes putatively involved in adventitious caulogenesis in P. pinea cotyledons was conducted. A multivariate analysis of both datasets was also realized through partial least squares regression and principal component analysis to obtain an integral vision of the mechanisms involved in caulogenesis in P. pinea. Analyses show that cotyledons cultured in the presence of benzyladenine during long times (2-6 days) cluster separately from the rest of the samples, suggesting that the benzyladenine increase observed during the first hours of culture is sufficient to trigger the caulogenic response through the activation of specific developmental programs. In particular, the most relevant factors involved in this process are the cytokinins trans-zeatin, dihydrozeatin, trans-zeatin riboside and isopentenyl adenosine; the auxin indoleacetic acid; and the genes PpWUS, PpWOX5, PpKN2, PpKN3 and PipiRR1. WUS is functional in pines and has an important role in caulogenesis. Interestingly, WOX5 also seems to participate in the process, although its specific role has not been determined.

中文翻译:

松果子叶不定茎发生过程中从头芽分生组织形成的激素和基因动力学。

WOX 和 KNOX 基因家族的几个成员和几个植物生长调节剂,主要是细胞分裂素和生长素,在针叶树 Pinus pinea 的不定茎发生过程中起关键作用。与拟南芥相似,松树芽器官发生是一个多步骤的过程。然而,这两个物种之间存在关键差异,这可能会改变潜在的生理和遗传程序。目前尚不清楚被子植物发育过程中的基因表达模型是否适用于针叶树。在这项工作中,分析了不同植物生长调节剂的内源含量和假定参与松果子叶不定茎发生的基因的表达。还通过偏最小二乘回归和主成分分析对这两个数据集进行了多变量分析,以全面了解 P. pinea 的茎发生机制。分析表明,长时间(2-6 天)在苄基腺嘌呤存在下培养的子叶与其余样品分开聚集,这表明在培养的最初几个小时内观察到的苄基腺嘌呤增加足以通过激活引发 caulogenic 反应具体的发展计划。特别是,与该过程最相关的因素是细胞分裂素反式玉米素、二氢玉米素、反式玉米素核苷和异戊烯基腺苷;生长素吲哚乙酸;以及基因 PpWUS、PpWOX5、PpKN2、PpKN3 和 PipiRR1。WUS 在松树中具有功能,并且在茎形成中具有重要作用。有趣的是,WOX5 似乎也参与了这个过程,尽管它的具体角色尚未确定。
更新日期:2020-01-30
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