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Identification and sex-biased profiles of candidate olfactory genes in the antennal transcriptome of the parasitoid wasp Cotesia vestalis.
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 3 ) Pub Date : 2020-01-29 , DOI: 10.1016/j.cbd.2020.100657
Yipeng Liu 1 , Lixiao Du 2 , Yun Zhu 2 , Shiyong Yang 3 , Qiong Zhou 4 , Guirong Wang 2 , Yang Liu 2
Affiliation  

Cotesia vestalis (Hymenoptera: Braconidae) is an endoparasitoid wasp that attacks larvae of the diamondback moth, Plutella xylostella (Linnaeus), one of the most important pests of cruciferous plants in the world. This natural enemy has been used to control populations of P. xylostella. To more fully understand the molecular mechanisms of olfactory reception in C. vestalis, the transcriptomes of male antennae, female antennae, and mixed female and male legs were systematically analyzed, and the chemosensory genes were identified. A total of 253 olfactory genes, including candidates of 158 odorant receptors (ORs), 12 ionotropic receptors (IRs), 48 gustatory receptors (GRs), 22 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), and two sensory neuron membrane proteins (SNMPs), were systematically identified and subjected to sequence and phylogenetic analysis. Then, the differentially expressed genes (DEGs) within female and male antennae were analyzed to obtain expression information on the candidate olfactory genes at the transcriptional level. The expression levels of 25 ORs, nine OBPs, eight GRs, and three IRs were significantly different between male and female antennae. Our results provide valuable information for further studies on the chemoreception mechanisms in C. vestalis, to ultimately improve the pest control measures by using natural enemies.

中文翻译:

寄生拟蜂黄蜂Cotesiavesalis触角转录组中候选嗅觉基因的鉴定和性别偏向分析。

Cotesiavestalis(Hymenoptera:Braconidae)是一种内寄生性黄蜂,会攻击小菜蛾幼虫小菜蛾(Plutella xylostella)(Linnaeus),后者是十字花科植物中最重要的害虫之一。这种天敌已被用来控制小菜蛾的种群。为了更全面地了解马鞭草嗅觉接收的分子机制,系统地分析了雄性触角,雌性触角以及雌雄混合的腿的转录组,并鉴定了化学感应基因。总共253个嗅觉基因,包括158个气味受体(OR),12个离子受体(IR),48个味觉受体(GR),22个气味结合蛋白(OBP),11个化学感觉蛋白(CSP)和两个感觉神经元候选物膜蛋白(SNMPs),被系统地鉴定,并进行序列和系统发育分析。然后,分析雌性和雄性触角内的差异表达基因(DEG),以获得转录水平上候选嗅觉基因的表达信息。雄性和雌性触角之间的25个OR,9个OBP,8个GR和3个IR的表达水平显着不同。我们的结果为进一步研究C.vestalis的化学感受机制提供有价值的信息,以最终通过使用天敌来改善害虫防治措施。男性和女性触角之间的三个IR显着不同。我们的结果为进一步研究C.vestalis的化学感受机制提供有价值的信息,以最终通过使用天敌来改善害虫防治措施。男性和女性触角之间的三个IR显着不同。我们的结果为进一步研究C.vestalis的化学感受机制提供有价值的信息,以最终通过使用天敌来改善害虫防治措施。
更新日期:2020-01-29
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