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Flux balance analysis of metabolism during growth by osmotic cell expansion and its application to tomato fruits.
The Plant Journal ( IF 7.2 ) Pub Date : 2020-01-27 , DOI: 10.1111/tpj.14707
Sanu Shameer 1 , José G Vallarino 2 , Alisdair R Fernie 2 , R George Ratcliffe 1 , Lee J Sweetlove 1
Affiliation  

Cell expansion is a significant contributor to organ growth and is driven by the accumulation of osmolytes to increase cell turgor pressure. Metabolic modelling has the potential to provide insights into the processes that underpin osmolyte synthesis and transport, but the main computational approach for predicting metabolic network fluxes, flux balance analysis, often uses biomass composition as the main output constraint and ignores potential changes in cell volume. Here we present growth‐by‐osmotic‐expansion flux balance analysis (GrOE‐FBA), a framework that accounts for both the metabolic and ionic contributions to the osmotica that drive cell expansion, as well as the synthesis of protein, cell wall and cell membrane components required for cell enlargement. Using GrOE‐FBA, the metabolic fluxes in dividing and expanding cells were analysed, and the energetic costs for metabolite biosynthesis and accumulation in the two scenarios were found to be surprisingly similar. The expansion phase of tomato fruit growth was also modelled using a multiphase single‐optimization GrOE‐FBA model and this approach gave accurate predictions of the major metabolite levels throughout fruit development, as well as revealing a role for transitory starch accumulation in ensuring optimal fruit development.

中文翻译:

渗透细胞膨胀作用下生长过程中新陈代谢的通量平衡分析及其在番茄果实中的应用。

细胞膨胀是器官生长的重要因素,并由渗透压的积累驱动,以增加细胞膨胀压力。代谢建模可能提供深入了解渗透压合成和运输过程的潜能,但预测代谢网络通量的主要计算方法(通量平衡分析)经常使用生物量成分作为主要输出约束,而忽略了细胞体积的潜在变化。在这里,我们介绍通过渗透-膨胀-通量平衡分析(GrOE-FBA)的框架,该框架说明了代谢和离子对推动细胞扩张以及蛋白质,细胞壁和细胞合成的渗透作用细胞扩大所需的膜成分。使用GrOE‐FBA,分析了分裂和扩增细胞中的代谢通量,两种情况下代谢物生物合成和积累的能源成本惊人地相似。还使用多阶段单优化GrOE-FBA模型对番茄果实生长的扩展阶段进行了建模,这种方法可以准确预测整个果实发育过程中的主要代谢产物水平,并揭示暂时性淀粉积累在确保最佳果实发育中的作用。 。
更新日期:2020-01-27
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