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Electrical impedance as an indicator of microalgal cell health.
Scientific Reports ( IF 4.6 ) Pub Date : 2020-01-27 , DOI: 10.1038/s41598-020-57541-6
Jianye Sui 1 , Fatima Foflonker 2 , Debashish Bhattacharya 2 , Mehdi Javanmard 1
Affiliation  

Separating specific cell phenotypes from a heterotypic mixture is a critical step in many research projects. Traditional methods usually require a large sample volume and a complex preparation process that may alter cell property during the sorting process. Here we present the use of electrical impedance as an indicator of cell health and for identifying specific microalgal phenotypes. We developed a microfluidic platform for measuring electrical impedance at different frequencies using the bacterium-sized green alga Picochlorum SE3. The cells were cultured under different salinity conditions and sampled at four different time points. Our results demonstrate the utility of electrical impedance as an indicator of cell phenotype by providing results that are consistent with known changes in cell size and physiology. Outliers in the cell data distribution are particularly useful because they represent phenotypes that have the ability to maintain size and/or membrane ionic permeability under prolonged salt stress. This suggests that our device can be used to identify and sort desired (e.g., experimentally evolved, mutant) cell phenotypes based on their electrical impedance properties.

中文翻译:

电阻抗是微藻细胞健康的指标。

从异型混合物中分离特定的细胞表型是许多研究项目中的关键步骤。传统方法通常需要较大的样品量和复杂的制备过程,这可能会在分选过程中改变细胞特性。在这里,我们介绍了使用电阻抗作为细胞健康状况的指标并用于识别特定的微藻表型。我们开发了一种微流体平台,用于使用细菌大小的绿藻Picochlorum SE3测量不同频率下的电阻抗。在不同盐度条件下培养细胞,并在四个不同时间点取样。我们的结果通过提供与已知的细胞大小和生理变化一致的结果,证明了电阻抗作为细胞表型指标的效用。细胞数据分布中的异常值特别有用,因为它们表示在延长的盐胁迫下具有维持大小和/或膜离子渗透性的能力的表型。这表明我们的设备可用于基于其电阻抗特性来识别和分类所需的(例如,实验发展,突变的)细胞表型。
更新日期:2020-01-27
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