As increasing demand of global reuse water, microbial desalination cell (MDC) is developed as a potential desalination approach to drive ion migration and separation through biodegradation without any additional energy. A novel, efficient, stable reactor coupled stacked MDC with upflow MDC was constructed, which was named as upflow-stacked MDC (USMDC). Compared with the traditional stacked MDC and upflow MDC, the desalination and generation performance of the USMDC was evaluated. Results showed that, after 24 h, the desalination ratio of USMDC can reach 91.9% when the external resistance was 1.5 Ω, which was 1.18 and 1.48 times higher than SMDC and UMDC, respectively. The long-term performance of the desalination efficiency was tested, which was maintained at 87.2–96.0% and stable for consecutive 120 days. Then, it was also the investigated that the relationship between desalination rate and external resistance during every period. The USMDC produced a maximum power density of 32.91 W m⁻³. In addition, the difference of current density between USMDC and SMDC indicates the turbulence generated by cylindrical structure could effectively decrease the internal resistance. It was also corroborated that salt concentration gradient and bipolar electrodialysis would decline the charge transfer efficiency. Accordingly, USMDC was verified having the superior desalination performance thus providing the possibility for application in wastewater reuse.

随着全球对水的再利用需求的增加，微生物脱盐池（MDC）被​​开发为一种潜在的脱盐方法，可通过生物降解来驱动离子迁移和分离，而无需任何额外的能量。构造了一种新颖，高效，稳定的带有上流MDC的堆叠MDC耦合反应堆，称为上流堆叠MDC（USMDC）。与传统的堆叠式MDC和上流式MDC相比，评估了USMDC的脱盐和发电性能。结果表明，在24 h后，当外部电阻为1.5Ω时，USMDC的脱盐率可达到91.9％，分别是SMDC和UMDC的1.18和1.48倍。测试了脱盐效率的长期性能，该性能保持在87.2–96.0％，并且连续120天保持稳定。然后，还研究了每个时期的淡化率和外部电阻之间的关系。USMDC产生的最大功率密度为32.91 W m⁻³。此外，USMDC和SMDC之间的电流密度差异表明，圆柱结构产生的湍流可以有效降低内部电阻。还证实了盐浓度梯度和双极电渗析会降低电荷转移效率。因此，USMDC被证实具有优异的脱盐性能，从而为废水回用提供了可能性。