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Toxicity of multi-wall carbon nanotubes inhalation on the brain of rats.
Environmental Science and Pollution Research ( IF 5.8 ) Pub Date : 2020-01-27 , DOI: 10.1007/s11356-020-07740-5
Fatemeh Samiei 1 , Farshad Hosseini Shirazi 1, 2 , Parvaneh Naserzadeh 1 , Faezeh Dousti 1 , Enayatollah Seydi 3, 4 , Jalal Pourahmad 1
Affiliation  

This study was designed to investigate the brain toxicity following the respiratory contact with multi-wall carbon nanotubes (MWCNTs) in male Wistar rats. Rats were exposed to 5 mg/m3 MWCNT aerosol in different sizes and purities for 5 h/day, 5 days/week for 2 weeks in a whole-body exposure chamber. After 2-week exposure, mitochondrial isolation was performed from different parts of rat brain (hippocampus, frontal cortex, and cerebellum) and parameters of mitochondrial toxicity including mitochondrial succinate dehydrogenase (SDH) activity, generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, mitochondrial swelling, and cytochrome c release, ATP level, mitochondrial GSH, and lipid peroxidation were evaluated. Our results demonstrated that MWCNTs with different characteristics, in size and purity, significantly (P < 0.05) decreased SDH activity, GSH, and ATP level, and increased mitochondrial ROS production, lipid peroxidation, mitochondrial swelling, MMP collapse, and cytochrome c release in the brain mitochondria. In conclusion, we suggested that MWCNTs with different characteristics, in size and purity, induce damage in varying degrees on the mitochondrial respiratory chain and increase mitochondrial ROS formation in different parts of rat brain (hippocampus, frontal cortex, and cerebellum).

中文翻译:

吸入多壁碳纳米管对大鼠大脑的毒性。

这项研究旨在研究雄性Wistar大鼠与多壁碳纳米管(MWCNT)呼吸接触后的脑毒性。在全身暴露室内,将大鼠暴露于5 mg / m3不同大小和纯度的MWCNT气雾剂5小时/天,5天/周,持续2周。暴露2周后,从大鼠大脑的不同部位(海马,额叶皮层和小脑)进行线粒体分离,并测定线粒体毒性参数,包括线粒体琥珀酸脱氢酶(SDH)活性,活性氧(ROS),线粒体膜的生成评估电位(MMP)崩溃,线粒体肿胀和细胞色素c释放,ATP水平,线粒体GSH和脂质过氧化作用。我们的结果表明,具有不同特性,尺寸和纯度的MWCNT,显着(P <0.05)降低SDH活性,GSH和ATP水平,并增加线粒体ROS的产生,脂质过氧化,线粒体肿胀,MMP塌陷和脑线粒体中细胞色素c的释放。总之,我们认为具有不同特征,大小和纯度的MWCNTs在大鼠大脑的不同部位(海马,额叶皮层和小脑)不同程度地诱导线粒体呼吸链损伤并增加线粒体ROS的形成。
更新日期:2020-01-27
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