Abstract Bacterial-associated infection and poor osseointegration usually led to the failure of titanium (Ti)-based in orthopedic implantation. To solve above issues, a bioactive coating consisted of gelatin (Gel) and chitosan-graft-polyaniline (Chi-PANI) was fabricated with layer-by-layer (LBL) assembly technique on titanium dioxide nanotubes (TNT) substrates. Scanning electron microscopy (SEM), water contact angle (WCA) measurement X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) results confirmed that the multilayers coating was successfully formed on TNT substrates. Antibacterial assay proved that the prepared sample (TNT-LBL (PANI)) had stronger ability against the adhesion and growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Besides, the prepared substrate was beneficial for osteoblast adhesion, proliferation and differentiation. Thus, these results shed light on the biocompatibility of PANI in enhancing antibacterial property and osteoblast activity for potential orthopedic application.

中文翻译：

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在钛基底上制备壳聚糖-移植物-聚苯胺基多层膜以增强抗菌性能和改善成骨活性

摘要 细菌相关感染和骨整合不良通常导致钛（Ti）基骨科植入失败。为了解决上述问题，采用逐层（LBL）组装技术在二氧化钛纳米管（TNT）基材上制备了由明胶（Gel）和壳聚糖接枝聚苯胺（Chi-PANI）组成的生物活性涂层。扫描电子显微镜 (SEM)、水接触角 (WCA) 测量 X 射线光电子能谱 (XPS) 和原子力显微镜 (AFM) 结果证实多层涂层已成功形成在 TNT 基材上。抗菌试验证明，制备的样品（TNT-LBL（PANI））对大肠杆菌（E.coli）和金黄色葡萄球菌（S.aureus）的粘附和生长具有较强的抑制能力。除了，制备的基质有利于成骨细胞的粘附、增殖和分化。因此，这些结果阐明了 PANI 在增强抗菌性能和成骨细胞活性方面的生物相容性，以用于潜在的骨科应用。