当前位置: X-MOL 学术Mol. Ther. Nucl. Acids › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition.
Molecular Therapy - Nucleic Acids ( IF 8.8 ) Pub Date : 2020-01-25 , DOI: 10.1016/j.omtn.2020.01.020
Jun-Qi Liu 1 , Ming Deng 2 , Nan-Nan Xue 1 , Ting-Xuan Li 1 , Yue-Xin Guo 1 , Liang Gao 3 , Di Zhao 4 , Rui-Tai Fan 1
Affiliation  

Esophageal squamous cell carcinoma (ESCC) is a common cancer occurring in males and females worldwide. Accumulating evidence continues to highlight the crucial roles of long non-coding RNAs (lncRNAs) in the process of tumorigenesis. However, the regulatory mechanism of lncRNAs in ESCC remains unclear. The aim of this study is to elucidate the role of lncRNA Krüppel-like factor 3 antisense RNA 1 (KLF3-AS1) in ESCC by regulating miR-185-5p and KLF3. Initially, ESCC cell spheres with stem cell-like properties were prepared by suspension culture, and subsequently characterized by assessing colony formation ability and stem cell markers. LncRNA KLF3-AS1 was found to be poorly expressed in ESCC and could upregulate the expression of KLF3 by binding to miR-185-5p. lncRNA KLF3-AS1 upregulation was observed to inhibit miR-185-5p, thereby contributing to decreased expression of SOX2 and Oct4 (octamer-binding transcription factor 4). Furthermore, enhancement of lncRNA KLF3-AS1 resulted in reduced colony formation ability, cell invasion and migration, and tumor volume in vivo while promoting cell apoptosis in ESCC through downregulation of miR-185-5p. Collectively, this study indicated that lncRNA KLF3-AS1 inhibited ESCC cell invasion and migration by impairing miR-185-5p-mediated inhibition of KLF3, highlighting a promising novel potential target for ESCC treatment.



中文翻译:

lncRNA KLF3-AS1通过损害miR-185-5p靶向的KLF3抑制来抑制ESCC中的细胞迁移和侵袭。

食道鳞状细胞癌(ESCC)是一种常见的癌症,在世界各地的男性和女性中均会发生。越来越多的证据继续突出了长非编码RNA(lncRNA)在肿瘤发生过程中的关键作用。但是,尚不清楚lncRNA在ESCC中的调控机制。这项研究的目的是通过调节miR-185-5p和KLF3阐明lncRNAKrüppel样因子3反义RNA 1(KLF3-AS1)在ESCC中的作用。最初,通过悬浮培养制备具有干细胞样特性的ESCC细胞球,然后通过评估菌落形成能力和干细胞标志物来表征。发现LncRNA KLF3-AS1在ESCC中表达较差,并可能通过与miR-185-5p结合而上调KLF3的表达。观察到lncRNA KLF3-AS1上调抑制了miR-185-5p,从而导致SOX2和Oct4(八聚体结合转录因子4)的表达降低。此外,lncRNA KLF3-AS1的增强导致集落形成能力,细胞侵袭和迁移以及肿瘤体积降低体内同时通过的miR-185-5p的下调促进细胞凋亡的ESCC。总体而言,这项研究表明,lncRNA KLF3-AS1通过削弱miR-185-5p介导的KLF3抑制作用来抑制ESCC细胞的侵袭和迁移,突显了有望用于ESCC治疗的新型潜在靶标。

更新日期:2020-01-25
down
wechat
bug