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Diffusion-limited biosensing of dissolved oxygen by direct electron transfer-type bioelectrocatalysis of multi-copper oxidases immobilized on porous gold microelectrodes
Journal of Electroanalytical Chemistry ( IF 4.5 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.jelechem.2020.113895
Masahiro Miyata , Yuki Kitazumi , Osamu Shirai , Kunishige Kataoka , Kenji Kano

Abstract Diffusion-controlled amperometric biosensors for dissolved oxygen (O2) were constructed by immobilization of multi-copper oxidases (copper efflux oxidase and bilirubin oxidase) on porous gold microdisk electrodes fabricated by anodization in a glucose solution. The immobilized enzymes rapidly consumed O2 near the electrode at potentials more negative than 0.2 V vs. Ag|AgCl|sat. KCl via direct electron transfer-type bioelectrocatalysis and the reduction current reached the steady state limiting value under static conditions. The fabricated biosensor exhibited a linear response to dissolved O2 concentration and was almost identical to the theoretical sensor, based on nonlinear diffusion of O2 around the microdisk electrode. The biosensor response was fast enough to monitor the catalytic consumption of dissolved O2 by glucose oxidase and exhibited storage stability for more than six days.

中文翻译:

通过固定在多孔金微电极上的多铜氧化酶的直接电子转移型生物电催化对溶解氧的扩散限制生物传感

摘要 通过在葡萄糖溶液中阳极氧化制备的多孔金微盘电极上固定多种铜氧化酶(铜流出氧化酶和胆红素氧化酶),构建了用于溶解氧 (O2) 的扩散控制电流生物传感器。固定化酶在电极附近迅速消耗 O2,其电位比 Ag|AgCl|sat 更负 0.2 V。KCl通过直接电子转移型生物电催化,在静态条件下还原电流达到稳态极限值。基于微盘电极周围 O2 的非线性扩散,制造的生物传感器对溶解的 O2 浓度表现出线性响应,并且与理论传感器几乎相同。
更新日期:2020-03-01
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