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Immunological and oxidative stress biomarkers in Ankylosing Spondylitis patients with or without metabolic syndrome
Cytokine ( IF 3.8 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.cyto.2020.155002
Alireza Pishgahi 1 , Rozita Abolhasan 2 , Shahla Danaii 3 , Bahareh Amanifar 2 , Mohammad Sadegh Soltani-Zangbar 4 , Majid Zamani 5 , Amin Kamrani 5 , Farzaneh Ghorbani 6 , Amir Mehdizadeh 7 , Hossein Samadi Kafil 8 , Farhad Jadidi-Niaragh 6 , Bahman Yousefi 8 , Mehrzad Hajialiloo 2 , Mehdi Yousefi 9
Affiliation  

Ankylosing Spondylitis (AS) is a chronic inflammatory disorder of the spine and sacroiliac joints with unidentified etiology closely associated with metabolic syndrome (MetS). Recent studies have reported that immunological and oxidative stress factors are implicated in AS pathogenesis. The aim of this study was to investigate the oxidative and immunological factors in AS patients with or without MetS compare to control group. Real-Time PCR measured expression level of cytokines, transcription factors and related miRNAs. In addition, Th17 and Treg frequencies and cytokines secretion were evaluated by flowcytometry and ELISA methods, respectively. The oxidative stress biomarkers were also assessed with biochemical methods. In AS patients with MetS, higher Th17 and lower Treg frequency were observed. Increased levels of NF-kB and AP-1 mRNA expression were seen in AS patients with MetS (p = 0.0263 and p = 0.0104, respectively). MiR-146a and miR-223 were significantly decreased (p = 0.0005, p = 0.0161, respectively) and increase in miR-21 (p = 0.0002) was observed in AS patients with MetS compared to AS patients without MetS. Additionally, the secretion of TNF-α (p = 0.0167), IL-1β (p = 0.303), CCL2 (p = 0.0254), CCL3 (p = 0.0119), CXCL8 (p = 0.0364), adiponectin (p = 0.0183) and the levels of SOD (p = 0.0421), NO (p = 0.0451) and CAT (p = 0.0128) were increased in AS patients with MetS. We were not observed significant differences in TOS and GPX levels between studied groups. The higher levels of oxidative stress and immunological inflammatory markers in AS patients with MetS provide further evidences on the oxidative stress and immunological relationship in these patients.

中文翻译:

伴有或不伴有代谢综合征的强直性脊柱炎患者的免疫和氧化应激生物标志物

强直性脊柱炎(AS)是脊柱和骶髂关节的慢性炎症性疾病,病因不明,与代谢综合征(MetS)密切相关。最近的研究报道了免疫和氧化应激因素与 AS 发病机制有关。本研究的目的是调查有或没有 MetS 的 AS 患者与对照组相比的氧化和免疫学因素。实时 PCR 测量细胞因子、转录因子和相关 miRNA 的表达水平。此外,分别通过流式细胞术和 ELISA 方法评估 Th17 和 Treg 频率和细胞因子分泌。氧化应激生物标志物也用生化方法进行了评估。在患有 MetS 的 AS 患者中,观察到较高的 Th17 和较低的 Treg 频率。在患有 MetS 的 AS 患者中观察到 NF-kB 和 AP-1 mRNA 表达水平增加(分别为 p = 0.0263 和 p = 0.0104)。与没有 MetS 的 AS 患者相比,在患有 MetS 的 AS 患者中观察到 MiR-146a 和 miR-223 显着降低(分别为 p = 0.0005、p = 0.0161)并且 miR-21 增加(p = 0.0002)。此外,TNF-α (p = 0.0167)、IL-1β (p = 0.303)、CCL2 (p = 0.0254)、CCL3 (p = 0.0119)、CXCL8 (p = 0.0364)、脂联素 (p = 0.018) 的分泌在患有 MetS 的 AS 患者中,SOD (p = 0.0421)、NO (p = 0.0451) 和 CAT (p = 0.0128) 的水平增加。我们没有观察到研究组之间 TOS 和 GPX 水平的显着差异。
更新日期:2020-04-01
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