Nitrogen and sulfur co-doped carbon dots (N,S-CDs) have been widely studied with high quantum yield (QY). The experimental conditions of three different N,S-CDs were optimized. Emission peak position of three different N,S-CDs shown almost remains unchanged or obvious excitation-dependent PL properties, that was likely owed to size distribution. In order to discuss the N,S-CDs stability of photoluminescence property in environment, various experiments such as the photostability, different pH, ionic strengths and temperature were designed. To sum up, three different N,S-CDs exhibited discrepancy property. Molecular interaction of three different N,S-CDs were produced via vary carbon source with human serum albumins have been investigate by various methods. The quenching mechanism, thermodynamic and kinetic parameters, binding sites, electrochemical behavior of three different N,S-CDs with human serum albumins have some different, but conformational change of three different N,S-CDs with human serum albumins alike. The molecular docking had successful applied to study the N,S-CDs interaction with HSA. Different N,S-CDs possessed various characteristic that will have different quenching mechanism when they interaction with human serum albumin, study the mechanism of action at molecular level will help people to choose suitable CDs to apply in nanomedical.

氮和硫共掺杂碳点（N，S-CDs）已被广泛研究，具有高量子产率（QY）。优化了三种不同N，S-CD的实验条件。所示的三种不同的N，S-CD的发射峰位置几乎保持不变或明显的依赖于激发的PL特性，这很可能是由于尺寸分布所致。为了讨论N，S-CDs在环境中的光致发光性质的稳定性，设计了各种实验，例如光稳定性，不同的pH，离子强度和温度。综上所述，三种不同的N，S-CD表现出差异性。已经通过各种方法研究了通过不同碳源与人血清白蛋白产生的三种不同N，S-CD的分子相互作用。淬灭机理，热力学和动力学参数，结合位点，三种不同的N，S-CD与人血清白蛋白的电化学行为有一些不同，但三种不同的N，S-CD与人血清白蛋白的构象变化相同。分子对接已成功应用于研究N，S-CD与HSA的相互作用。不同的N，S-CD与人血清白蛋白相互作用时具有不同的猝灭机制，研究分子水平的作用机制将有助于人们选择合适的CD应用于纳米医学。