当前位置: X-MOL 学术Fish Shellfish Immun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Edwardsiella tarda induces enteritis in farmed seahorses (Hippocampus erectus): An experimental model and its evaluation.
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-01-25 , DOI: 10.1016/j.fsi.2020.01.049
Xiaomeng Wang 1 , Fang Wang 2 , Guozhong Chen 1 , Boya Yang 3 , Jun Chen 3 , Yan Fang 3 , Kai Wang 3 , Yuping Hou 1
Affiliation  

Bacterial enteritis is an important deadly threat to farmed seahorses. However, its pathogenesis is obscure because of the paucity of reproducible experimental intestinal inflammation models. Herein, a strain of Edwardsiella tarda YT1 from farmed seahorse Hippocampus erectus was isolated and identified by morphological, phylogenetic, and biochemical analysis, and confirmed as a pathogen of enteritis for the first time by challenge experiment. Two E. tarda concentrations (1 × 105 and 1 × 107 colony forming units [cfu] ml-1) were confirmed suitable for an enteritis model by intraperitoneal injection. To develop and evaluate the experimental model, we challenged seahorses with E. tarda and found that (1) the infection inhibited body length increase, significantly decreased body weight (P < 0.05), and induced typical pathological features including anorexia, anal inflammation, and intestinal fluid retention; (2) 19 external (weight, height, anal inflammation, feeding status, and intestinal fluid retention), histological (goblet and inflammatory cell numbers and thickening of lamina propria and muscularis mucosae), and molecular (hepcidin, liver-expressed antimicrobial peptide, lysozyme, piscidin, interleukin [IL]-1β, IL-1β receptor, IL-2, IL-10, interferon1, tumor necrosis factor [TNF]-α, and toll-like receptor 5 [TLR5]) indicators were suitable for model evaluation, as they could sensitively respond and varied similarly throughout the experiment, indicating the high sensitivity of seahorses against pathogen invasion; (3) TLR5 may play an essential role in triggering host immune responses during E. tarda-induced chronic enteritis, and (4) the evaluating system could reflect the pattern and intensity of disease progression. Thus, we developed an experimental model and an evaluating system of bacterial enteritis in farmed seahorses, helping us to reveal the pathogenesis of bacterial enteritis, identify potential therapeutic drugs, and search suitable genetic markers for seahorse molecular breeding.

中文翻译:

泰氏爱德华氏菌在养殖的海马中引起肠炎:一种实验模型及其评价。

细菌性肠炎是对养殖海马的重要致命威胁。然而,由于可重复的实验性肠道炎症模型的缺乏,其发病机理是不清楚的。在此,通过形态,系统发育和生化分析分离并鉴定了养殖的海马直立海马中的爱德华氏菌YT1菌株,并通过攻击实验首次确认其为肠炎病原体。通过腹膜内注射证实两种浓度的塔氏大肠杆菌(1×105和1×107菌落形成单位cml-1)适合于肠炎模型。为了开发和评估实验模型,我们用大肠杆菌感染了海马,发现(1)感染抑制了体长的增加,体重显着降低(P <0.05),并诱发典型的病理特征,包括厌食,肛门发炎和肠液retention留;(2)19个外部(体重,身高,肛门发炎,进食状态和肠道液体滞留),组织学(杯状和炎性细胞数量以及固有层和肌层粘膜增厚)和分子(肝素,肝表达的抗菌肽),溶菌酶,piscidin,白介素[IL]-1β,IL-1β受体,IL-2,IL-10,干扰素1,肿瘤坏死因子[TNF]-α和Toll样受体5 [TLR5])指标适用于模型评估,因为它们在整个实验过程中都可以灵敏地做出反应并且变化类似,这表明海马对病原体入侵具有很高的敏感性;(3)在E. tarda引起的慢性肠炎期间,TLR5可能在触发宿主免疫反应中起重要作用,(4)评价体系可以反映疾病进展的方式和强度。因此,我们开发了养殖海马中细菌性肠炎的实验模型和评估系统,帮助我们揭示了细菌性肠炎的发病机制,确定了潜在的治疗药物,并寻找了适合海马分子育种的遗传标记。
更新日期:2020-01-26
down
wechat
bug