Two undescribed bufadienolide glucosides and four undescribed bufadienolides were isolated from the whole plants of Helleborus foetidus (Ranunculaceae). Their structures were determined by extensive spectroscopic analysis and the results of hydrolytic cleavage. The isolated compounds exhibited cytotoxic activities against HL-60 and A549 cells with IC50 values ranging from 0.019 to 3.0 μM. The isolated compounds also showed the Na+/K+ ATPase inhibitory activity. The undescribed compound 16β-formyloxy-10,14-dihydroxy-5β-[(β-d-glucopyranosyl)oxy]-19-norbufa-3,20,22-trienolide induced apoptosis in HL-60 cells through a mitochondria-dependent apoptotic pathway.

中文翻译：

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臭椿全株蟾蜍二烯内酯及其细胞毒性

两种未描述的蟾蜍二烯内酯糖苷和四种未描述的蟾蜍二烯内酯是从全株 Helleborus foetidus（毛茛科）中分离出来的。它们的结构是通过广泛的光谱分析和水解裂解的结果确定的。分离的化合物对 HL-60 和 A549 细胞表现出细胞毒活性，IC50 值范围为 0.019 至 3.0 μM。分离的化合物还显示出 Na+/K+ ATPase 抑制活性。未描述的化合物 16β-formyloxy-10,14-dihydroxy-5β-[(β-d-glupyranosyl)oxy]-19-norbufa-3,20,22-trienolide 通过线粒体依赖性凋亡诱导 HL-60 细胞凋亡途径。