The intensive application of type-II pyrethroid worldwide in agricultural and residential practices potentially contributes to soil and water pollution, raising various concerns about environmental and public health. In the present study, robust fungus (strain PYR-P2) with high pyrethroids degradation potential was isolated from pesticide-contaminated soil. The strain was identified based on morphology and molecular characteristics, as Aspergillus sp. The screening of the transforming ability of strain PYR-P2 was evaluated in minimal salt media (MSM), where the fungus utilized up to 500 mg L-1 of pyrethroid mixture (cypermethrin (CYP), cyfluthrin (CYF), cyhalothrin (CYH)). With this in view, central composite design (CCD) with three independent variables (pH, temperature, and initial concentration) was employed to identify the optimal conditions for achieving maximum pyrethroid removal. Under optimal conditions, strain PYR-P2 was implemented for the bioaugmentation studies in natural and sterile soil (NS/SS) systems spiked with pyrethroid (single and mixture) at a concentration of 100 mg kg-1. The highest pyrethroid removal percentages were observed in fungally augmented NS, accompanied by a decrease in pyrethroid half-life (t1/2). Herein, the observed half-life (t1/2) of pyrethroids in the fungally augmented NS varied between 1.48 and 2.69 d, with equally good values recorded in SS as 1.65-3.10 d. Taken together, the mycoremediation study employing fungal (strain PYR-P2) augmentation under optimized conditions represents an efficient strategy to restore pyrethroid-contaminated soil.

中文翻译：

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有效的mycoremediation结合生物强化研究：对新分离的曲霉菌的高级研究。在II型拟除虫菊酯污染的土壤中。

II型拟除虫菊酯在世界范围内在农业和住宅实践中的广泛应用可能导致土壤和水污染，引起对环境和公共健康的各种担忧。在本研究中，从农药污染的土壤中分离出具有高拟除虫菊酯降解潜力的健壮真菌（菌株PYR-P2）。根据形态和分子特征鉴定该菌株为曲霉菌。在基本盐培养基（MSM）中评估了菌株PYR-P2转化能力的筛选，其中真菌利用了高达500 mg L-1的拟除虫菊酯混合物（氯氰菊酯（CYP），氟氰菊酯（CYF），氟氯氰菊酯（CYH）） ）。有鉴于此，中央复合设计（CCD）具有三个独立变量（pH，温度，和初始浓度）来确定实现最大拟除虫菊酯去除的最佳条件。在最佳条件下，将PYR-P2菌株用于天然和无菌土壤（NS / SS）系统中的生物强化研究，掺入拟除虫菊酯（单一和混合物）的浓度为100 mg kg-1。在真菌增强的NS中观察到了最高的拟除虫菊酯去除百分比，同时降低了拟除虫菊酯半衰期（t1 / 2）。在此，在真菌增强的NS中观察到的拟除虫菊酯的半衰期（t1 / 2）在1.48和2.69 d之间变化，在SS中记录的同样好的值为1.65-3.10 d。综上所述，在优化条件下采用真菌（菌株PYR-P2）扩增的mycoremediation研究代表了一种恢复拟除虫菊酯污染的土壤的有效策略。