Estimates of quantitative proteomic distance between populations have not been reported to date. Here, quantitative proteomic distances between three Spanish bovine breeds (Asturiana de los Valles, AV; Retinta, RE; and Rubia Gallega, RG) were estimated from two-dimensional electrophoresis profiles of meat samples of longissimus thoracis muscle at 2 h post-mortem. Statistically significant distances were detected between AV/RG and the most genetically different RE breed, using the novel QD measure of quantitative proteomic distance. In total, 18 differentially abundant myofibrillar and sarcoplasmic proteins/isoforms contributing to proteomic distances between breeds were confidently identified by tandem mass spectrometry. The fast skeletal myosin regulatory light chain 2 followed by other five interacting proteins exhibited the most pronounced relative change between breeds. In addition, most differentially represented proteins could be associated with variations in meat tenderness. Therefore, they could be candidate biomarkers for molecular breeding programs and authentication of the three Spanish beef breeds.

中文翻译：

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测量西班牙牛肉品种之间的定量蛋白质组距离。

迄今为止，尚未报道种群之间定量蛋白质组距离的估计。在这里，从死后2 h胸大肌长肌肉样品的二维电泳图谱中估计了三个西班牙牛品种（阿斯图里亚纳·德·洛斯·瓦雷斯，AV； Retinta，RE；和Rubia Gallega，RG）之间的定量蛋白质组距离。使用新颖的定量蛋白质组距离QD量度，在AV / RG与遗传上最不相同的RE品种之间检测到统计学上显着的距离。通过串联质谱法，总共可以确定地鉴定出18个差异丰富的肌原纤维和肌浆蛋白/同工型，这些蛋白/同工型有助于品种之间的蛋白质组学距离。快速骨骼肌肌球蛋白调节性轻链2，然后是其他五个相互作用的蛋白，在品种之间表现出最明显的相对变化。此外，大多数差异表达的蛋白质可能与肉嫩度的变化有关。因此，它们可以作为分子育种计划和西班牙三个牛肉品种认证的候选生物标记。