Arbuscular mycorrhizal (AM) fungi are important to the establishment of native vegetation for mined land rehabilitation, particularly in semi-arid and infertile landscapes. However, the information has been scarce about the colonization of AM fungal community in alkaline magnetite Fe ore tailing sites (without toxic metal (loid) contamination). The present study has characterized the diversity of AM fungi across typical domains of a magnetite Fe ore mine located in 200 km south-east of Geraldton, Western Australia, by adopting high throughput Illumina Miseq sequencing. The investigated domains included two tailing sites without top soil covering (T1 and T2), a rehabilitated area of tailings with top soil covering (R1), a revegetated waste rock area (R2), and two native undisturbed soil sites (S1 and S2). The results indicated that the T1/T2 sites had different AM fungal community structure, compared with R1/R2 and S1/S2 sites. The dominant families were Glomeraceae, Claroideoglomeraceae, Archaeosporaceae, Ambisporaceae, and Paraglomeraceae, with Paraglomeraceae (more than 50%) as the most abundant in the T1/T2 and R1/R2 sites. At genus level, Ambispora spp. and Archaeospora spp. were rich in T1/T2 sites (> 10%), while Glomus spp. were preferably dominant in S1/S2 sites (> 10%). Furthermore, amorphous Fe and available P were found to explain the variations associated with AM fungal community composition, particularly the abundance of Archaeosporaceae and Glomeraceae. The study revealed the AM fungal community composition shift across the gradient of Fe ore mine sites, as well as the effects of revegetation on AM fungal community development. The findings indicate the possible restoration of AM fungal community in the tailings undergoing revegetation, and potential adoption of indigenous AM fungi to rapid phytostabilization of the Fe ore tailings under semi-arid climatic conditions.

中文翻译：

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碱性铁矿石尾矿梯度中丛生菌根真菌群落的分子多样性，重新植被了废石到天然土壤。

丛枝菌根（AM）真菌对于建立天然植物以恢复雷区，特别是在半干旱和不孕景观中非常重要。然而，关于AM真菌群落在碱性磁铁矿铁矿石尾矿位定居的信息很少（没有有毒金属（金属）污染）。本研究通过采用高通量Illumina Miseq测序，表征了位于澳大利亚西部杰拉尔顿东南200 km的磁铁矿铁矿石典型区域中AM真菌的多样性。研究范围包括两个没有顶面土壤覆盖的尾矿场（T1和T2），一个带有顶面土壤覆盖的尾矿恢复区（R1），一个植被恢复的waste石区（R2）和两个原生原状土壤站点（S1和S2）。 。结果表明，与R1 / R2和S1 / S2位点相比，T1 / T2位点具有不同的AM真菌群落结构。优势科是glomeraceae，Claroideoglomeraceae，Archaeosporaceae，Ambisporaceae和Paraglomeraceae，在T1 / T2和R1 / R2位点，伞形科（50％以上）最丰富。在属水平上，双翅目属。和古菌属。富含T1 / T2站点（> 10％），而Glomus物种。最好在S1 / S2位点占主导地位（> 10％）。此外，发现无定形的铁和有效磷可以解释与AM真菌群落组成有关的变异，特别是古细菌科和豆科植物的丰度。该研究揭示了AM真菌群落组成在整个铁矿石矿场的梯度范围内转移，以及植被恢复对AM真菌群落发展的影响。