5,6-Dihydroergosterol-glucose is an organic synthetic derivative of spinasterol-glucose, which has potent anti-inflammatory activity. We previously synthesized alpha and beta anomers of DHE-glycosides and compared their inhibitory activity on CCL17 and CCL22 mRNA expression induced by TNF-α/IFN-γ in activated HaCaTs. Recently, we synthesized a new type of DHE-glycosides, 3-epi-5,6-dihydroergosterol(3-epi-DHE)-glycosides, and compared its inhibitory activity on mRNA expression levels of CCL17 and CCL22 in TNF-α/IFN-γ-induced HaCaT. DHE-Xly did not affect TNF-α/IFN-γ induced CCL17 and CCL22 mRNA expression in HaCaTs, however, 3-epi-DHE-Xly strongly inhibited TNF-α/IFN-γ induced CCL17 and CCL22 mRNA expression levels in human keratinocytes. These results provide important clues for development of chronic dermatitis treatment via inhibition of chemokine expression using DHE derivatives.

中文翻译：

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5,6-二氢麦角甾醇和3-epi-5,6-二氢麦角甾醇对人角质形成细胞趋化因子表达影响的比较研究

5,6-二氢麦角甾醇葡萄糖是一种有机合成的菠菜甾醇葡萄糖衍生物，具有强效抗炎活性。我们之前合成了 DHE-糖苷的 alpha 和 beta 端基异构体，并比较了它们对活化 HaCaT 中由 TNF-α/IFN-γ 诱导的 CCL17 和 CCL22 mRNA 表达的抑制活性。最近，我们合成了一种新型的DHE-糖苷，3-epi-5,6-二氢麦角甾醇(3-epi-DHE)-糖苷，并比较了其对TNF-α/IFN中CCL17和CCL22 mRNA表达水平的抑制活性。 -γ 诱导的 HaCaT。DHE-Xly 不影响 HaCaTs 中 TNF-α/IFN-γ 诱导的 CCL17 和 CCL22 mRNA 表达，然而，3-epi-DHE-Xly 强烈抑制人角质形成细胞中 TNF-α/IFN-γ 诱导的 CCL17 和 CCL22 mRNA 表达水平.