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A Colorimetric and Fluorometric Polydiacetylene Biothiol Sensor Based on Decomposition of a Pyridine-Mercury Complex
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2020-01-24 , DOI: 10.1016/j.snb.2020.127771
Jong Pil Lee , Fadilatul Jannah , Kwangmin Bae , Jong-Man Kim

Biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) participate in numerous cellular functions and physiological processes. Because changes in the levels of these biological important substances are associated with various clinical disorders and diseases, methods for their selective sensitive and quantitative detection are in high demand. In the current study, we developed a new, selective and sensitive colorimetric and fluorometric biothiol chemosensor that is comprised of a mercury-complexed, pyridine-containing polydiacetylene (PDA). Owing to its high affinity toward sulfur, biothiols promote decomposition of the pyridine-mercury complex in the PDA in conjunction with colorimetric and fluorescence changes. Specifically, upon addition of biothiols the new chemosensor undergoes a blue-to-red color transition and generates red fluorescence. Moreover, incorporation of the chemosensor in electrospun PEO nanofibers leads to an enhanced sensitivity for biothiol detection. The strategy developed in this study should be applicable to the design of other thiol-specific sensors that utilize pyridine-containing conjugated molecules.



中文翻译:

基于吡啶-汞配合物分解的比色和荧光聚二乙炔生物硫醇传感器

半胱氨酸(Cys),高半胱氨酸(Hcy)和谷胱甘肽(GSH)等生物硫醇参与许多细胞功能和生理过程。由于这些生物重要物质的水平变化与各种临床病症和疾病有关,因此对它们的选择性灵敏和定量检测的方法提出了很高的要求。在当前的研究中,我们开发了一种新型的,选择性的和灵敏的比色和荧光生物硫醇化学传感器,该传感器由汞络合物,含吡啶的聚二乙炔(PDA)组成。由于其对硫的高亲和力,生物硫醇与比色和荧光变化一起促进了PDA中吡啶-汞配合物的分解。特别,添加生物硫醇后,新的化学传感器会经历蓝色到红色的过渡,并产生红色荧光。此外,在电纺PEO纳米纤维中加入化学传感器可提高生物硫醇检测的灵敏度。这项研究中开发的策略应适用于利用含吡啶共轭分子的其他巯基特异性传感器的设计。

更新日期:2020-01-24
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