In this work, we proposed the concept of fluorescent speed sensing (FSS) of glycoprotein in chip supramolecular electrophoresis (CSE). To achieve a stable FSS run, we firstly synthesized a novel boronic acid-based diben-zoxanthene receptor (Rp3) by a simple one-pot reaction and the complex probe of [email protected] containing Rp3 and fluorescein (F1), and performed a series of experiments to assess the complex probe's ability for recognition of saccharides and glycoproteins. Secondly, we revealed the mechanism of 'balance electromigration of supramolecular complex probe' in FSS and developed the relevant theory of FSS via a series of theoretical derivation. The FSS theory showed that the moving speed of F1 was a function of glycoprotein content, indicating a novel model of glycoprotein sensing. To demonstrate the validity and utility of developed model and theory, IgG and ovalbumin were selected as model glycoproteins for the relevant experiments. The results verified the validity of FSS concept to glycoprotein assay with good accuracy (less than 6.33% difference from standard HPLC method), stability (less than 6.04% RSD) and linearity (higher than R² = 0.99) as well as fair sensitivity (8.48 ng). Finally, the FSS was successfully used for the analysis of HbA_1c (a key biomarker in diabetes blood). The developed FSS concept and complex probe have great potential for glycoprotein sensing, particularly for the diagnosis of diabetes.

在这项工作中，我们提出了芯片超分子电泳（CSE）中糖蛋白的荧光速度传感（FSS）的概念。为了实现稳定的FSS运行，我们首先通过简单的一锅反应和包含Rp3和荧光素（F1）的[受电子邮件保护的]复杂探针合成了一种新型的基于硼酸的二苯并氧杂蒽受体（Rp3），并进行了评估复杂探针识别糖和糖蛋白的能力的一系列实验。其次，我们揭示了FSS中“超分子复合探针的平衡电迁移”的机理，并通过一系列理论推导发展了FSS的相关理论。FSS理论表明，F1的移动速度是糖蛋白含量的函数，表明糖蛋白传感的新模型。为了证明开发的模型和理论的有效性和实用性，选择了IgG和卵清蛋白作为相关实验的模型糖蛋白。结果证实了FSS概念对糖蛋白测定的正确性（与标准HPLC方法的差异小于6.33％），稳定性（RSD小于6.04％）和线性（高于R ²  = 0.99）以及中等灵敏度（8.48 ng）。最后，FSS已成功用于HbA _1c（糖尿病血液中的关键生物标志物）的分析。发达的FSS概念和复杂的探针在糖蛋白感测方面具有巨大潜力，尤其是对于糖尿病的诊断。