Polarization‐resolved second‐harmonic generation (P‐SHG) microscopy is a technique capable of characterizing nonlinear optical properties of noncentrosymmetric biomaterials by extracting the nonlinear susceptibility tensor components ratio , with z‐axis parallel and x‐axis perpendicular to the C₆ symmetry axis of molecular fiber, such as a myofibril or a collagen fiber. In this paper, we present two P‐SHG techniques based on incoming and outgoing circular polarization states for a fast extraction of : A dual‐shot configuration where the SHG circular anisotropy generated using incident right‐ and left‐handed circularly‐polarized light is measured; and a single‐shot configuration for which the SHG circular anisotropy is measured using only one incident circular polarization state. These techniques are used to extract the of myosin fibrils in the body wall muscles of Drosophila melanogaster larva. The results are in good agreement with values obtained from the double Stokes‐Mueller polarimetry. The dual‐ and single‐shot circular anisotropy measurements can be used for fast imaging that is independent of the in‐plane orientation of the sample. They can be used for imaging of contracting muscles, or for high throughput imaging of large sample areas.

中文翻译：

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在二次谐波产生显微镜中使用圆偏振进行双次和单次磁化率比测量。

偏振分辨二次谐波产生 (P-SHG) 显微镜是一种能够通过提取非线性磁化率张量比来表征非中心对称生物材料的非线性光学特性的技术，其中z轴平行，x轴垂直于 C ₆对称轴分子纤维，例如肌原纤维或胶原纤维。在本文中，我们提出了两种基于传入和传出圆极化状态的 P-SHG 技术，用于快速提取：测量使用入射右旋和左旋圆偏振光产生的 SHG 圆形各向异性的双镜头配置；以及仅使用一个入射圆偏振态测量 SHG 圆形各向异性的单次配置。这些技术用于提取黑腹果蝇幼虫体壁肌肉中的肌球蛋白原纤维。结果与从双 Stokes-Mueller 旋光仪获得的值非常一致。双次和单次圆形各向异性测量可用于独立于样品平面内方向的快速成像。它们可用于收缩肌肉的成像，或用于大样本区域的高通量成像。