Reactive Oxygen species (ROS) are well known for their oxidizing behaviour but it differs in fluorescein derivatives chemistry. With t‐BuOOH, the probe F shows its unique turn‐off sensing (A), while with NaOCl probe shows turn‐on sensing property (B). In this study, it was observed that in situ generated species formed by the reaction of probe (F) with t‐BuOOH is a unique molecule, having high turn‐on behaviour in presence of sodium hypochlorite (≈500 times). Further, fluorescence microscopy imaging using adenocarcinoma cells, A549 cells revealed that in‐situ generated molecule, could be used as an efficient fluorescent probe for detecting NaOCl in living cells.

中文翻译：

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“在肺腺癌细胞中原位生成的荧光素探针的“打开”传感行为及其次氯酸钠对氢过氧化叔丁基的优先选择性”

活性氧（ROS）的氧化行为众所周知，但荧光素衍生物的化学性质不同。对于t- BuOOH，探头F显示其独特的关闭感应（A），而对于NaOCl，探头F显示其开启感应特性（B）。在这项研究中，观察到由探针（F）与t- BuOOH反应形成的原位产生的物种是一个独特的分子，在次氯酸钠存在下具有高开启行为（≈500倍）。此外，使用腺癌细胞A549细胞的荧光显微镜成像显示，原位生成的分子可以用作检测活细胞中NaOCl的有效荧光探针。