The venom protein components of Malabar pit viper (Trimeresurus malabaricus) were identified by combining SDS-PAGE and ion-exchange chromatography pre-fractionation techniques with LC-MS/MS incorporating Novor and PEAKS-assisted de novo sequencing strategies. Total 97 proteins that belong to 16 protein families such as L-amino acid oxidase, metalloprotease, serine protease, phospholipase A2, 5'-nucleotidase, C-type lectins/snaclecs and disintegrin were recognized from the venom of a single exemplar species. Of the 97 proteins, eighteen were identified through de novo approaches. Immunological cross-reactivity assessed through ELISA and western blot indicate that the Indian antivenoms binds less effectively to Malabar pit viper venom components compared to that of Russell's viper venom. The in vitro cell viability assays suggest that compared to the normal cells, MPV venom induces concentration dependent cell death in various cancer cells. Moreover, crude venom resulted in chromatin condensation and apoptotic bodies implying the induction of apoptosis. Taken together, the present study enabled in dissecting the venom proteome of Trimeresurus malabaricus and revealed the immuno-cross-reactivity profiles of commercially available Indian polyvalent antivenoms that, in turn, is expected to provide valuable insights on the need in improving antivenom preparations against its bite.

中文翻译：

---

描绘印度西高止山脉的马拉巴尔毒蛇（Trimeresurus malabaricus）的毒毒素武库，并评估其免疫交叉反应性和体外细胞毒性。

通过结合SDS-PAGE和离子交换色谱预分离技术以及结合Novor和PEAKS辅助从头测序策略的LC-MS / MS，鉴定了马拉巴尔pit蛇（Trimeresurus malabaricus）的毒蛋白成分。可以从一个示例物种的毒液中识别出属于16个蛋白质家族的97种蛋白质，例如L-氨基酸氧化酶，金属蛋白酶，丝氨酸蛋白酶，磷脂酶A2、5'-核苷酸酶，C型凝集素/突触核蛋白和双整合蛋白。在97种蛋白质中，有18种是通过从头鉴定的。通过ELISA和Western印迹评估的免疫学交叉反应性表明，与Russell的毒蛇毒相比，印度抗蛇毒血清与马拉巴尔毒蛇毒蛇毒成分的结合效率较低。体外细胞生存力分析表明，与正常细胞相比，MPV毒液在多种癌细胞中诱导浓度依赖性细胞死亡。此外，粗毒导致染色质浓缩和凋亡小体，提示细胞凋亡。综上所述，本研究能够剖析马拉加隆氏小牛的蛇毒蛋白质组，并揭示了市售的印度多价抗蛇毒血清的免疫交叉反应谱，从而有望为改进抗蛇毒血清制备抗蛇毒血清提供有价值的见解。咬。