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Comprehensive identification of alternative back-splicing in human tissue transcriptomes.
Nucleic Acids Research ( IF 14.9 ) Pub Date : 2020-01-24 , DOI: 10.1093/nar/gkaa005
Peng Zhang 1 , Xiao-Ou Zhang 2 , Tingting Jiang 3 , Lingling Cai 1 , Xiao Huang 1 , Qi Liu 1 , Dan Li 1 , Aiping Lu 1 , Yan Liu 1 , Wen Xue 3 , Peng Zhang 1 , Zhiping Weng 1, 2
Affiliation  

Circular RNAs (circRNAs) are covalently closed RNAs derived from back-splicing of genes across eukaryotes. Through alternative back-splicing (ABS), a single gene produces multiple circRNAs sharing the same back-splice site. Although many ABS events have recently been discovered, to what extent ABS involves in circRNA biogenesis and how it is regulated in different human tissues still remain elusive. Here, we reported an in-depth analysis of ABS events in 90 human tissue transcriptomes. We observed that ABS occurred for about 84% circRNAs. Interestingly, alternative 5' back-splicing occurs more prevalently than alternative 3' back-splicing, and both of them are tissue-specific, especially enriched in brain tissues. In addition, the patterns of ABS events in different brain regions are similar to each other and are more complex than the patterns in non-brain tissues. Finally, the intron length and abundance of Alu elements positively correlated with ABS event complexity, and the predominant circRNAs had longer flanking introns and more Alu elements than other circRNAs in the same ABS event. Together, our results represent a resource for circRNA research-we expanded the repertoire of ABS events of circRNAs in human tissue transcriptomes and provided insights into the complexity of circRNA biogenesis, expression, and regulation.

中文翻译:

全面鉴定人类组织转录组中的其他反向剪接。

环状RNA(circRNA)是共价闭合的RNA,其来源于跨真核生物的基因反向剪接。通过可替代的反向剪接(ABS),单个基因可产生共享相同反向剪接位点的多个circRNA。尽管最近发现了许多ABS事件,但是ABS参与circRNA生物发生的程度以及如何在不同的人体组织中进行调节仍然是未知的。在这里,我们报道了90个人类组织转录组中ABS事件的深入分析。我们观察到大约84%的circRNA发生ABS。有趣的是,替代性5'反向剪接比替代性3'反向剪接更普遍,并且两者都是组织特异性的,尤其是富含脑组织。此外,不同大脑区域中ABS事件的模式彼此相似,并且比非脑组织中的模式更复杂。最后,内含子的长度和Alu元素的丰度与ABS事件的复杂性呈正相关,并且在同一ABS事件中,主要circRNA的侧翼内含子和Alu元素的数量比其他circRNA多。总之,我们的结果代表了circRNA研究的资源-我们扩大了人类组织转录组中circRNA的ABS事件库,并提供了对circRNA生物发生,表达和调控的复杂性的见解。在同一ABS事件中,主要circRNA的侧翼内含子更长,Alu元素也多于其他circRNA。总之,我们的结果代表了circRNA研究的资源-我们扩大了人类组织转录组中circRNA的ABS事件库,并提供了对circRNA生物发生,表达和调控的复杂性的见解。在同一ABS事件中,主要circRNA的侧翼内含子更长,Alu元素也多于其他circRNA。总之,我们的结果代表了circRNA研究的资源-我们扩大了人类组织转录组中circRNA的ABS事件库,并提供了对circRNA生物发生,表达和调控的复杂性的见解。
更新日期:2020-01-24
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