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Label-free analysis of adsorbed protein heterogeneity on individual particles, based on single particle collision events
Electrochemistry Communications ( IF 5.4 ) Pub Date : 2020-01-24 , DOI: 10.1016/j.elecom.2020.106666
Wei Yi , Cong Xu , Tianyi Xiong , Tienan Gao , Ping Yu , Xiaohua He , Lanqun Mao

Investigation of the heterogeneity of protein adsorption on particle surfaces has attracted enormous research attention owing to its great importance in fundamental studies and quality control. Herein we, for the first time, report a new method for label-free analysis of the heterogeneity of protein adsorption on single nanoparticles, based on particle collision events at the orifice of a nanopipette. The dwell time was strongly dependent on the amount of protein adsorbed on polystyrene particles, which could be used to analyze the heterogeneity of protein adsorption at the single particle level. This method presents a label-free, sensitive, reproducible and easily-operated way to analyze adsorption behavior at the single particle level, which opens a new approach to the study of the heterogeneity of physicochemical parameters at the surface of nanoparticles.



中文翻译:

基于单个粒子碰撞事件的无标记分析单个粒子上吸附的蛋白质异质性

蛋白质在颗粒表面吸附的异质性研究由于其在基础研究和质量控制中的重要性而备受关注。本文中,我们首次报告了一种基于纳米吸管孔口处的粒子碰撞事件的无标记分析蛋白质吸附在单个纳米粒子上的异质性的新方法。停留时间在很大程度上取决于吸附在聚苯乙烯颗粒上的蛋白质的量,可用于分析单个颗粒水平上蛋白质吸附的异质性。该方法提供了一种无标记,灵敏,可重现且易于操作的方式来分析单个粒子水平的吸附行为,

更新日期:2020-01-24
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