Abstract

Inosine monophosphate (IMP) was considered to be an important component of meat flavor and an important indicator for evaluating the quality of meat products. This paper developed a novel dual-enzyme biosensor for the quantitative detection of inosinic acid to assess meat quality. Using the conductivity of MXene materials and the ability of Au@Pt nanoflowers to catalyze the oxidation of H₂O₂, a dual-enzyme biosensor was assembled and prepared for sensitive and rapid detection of IMP. The MXene-Ti₃C₂Tx material had 2D nanostructure similar to that of graphene, as well as metal conductivity and good biocompatibility. MXene was used as a carrier for 5′-nucleotidase and xanthine oxidase, with good biological environment and stable microenvironment. Bimetallic nanoflowers with a core-shell structure had better ability to catalyze H₂O₂ than the single metal. The double-enzyme hydrolyzed IMP to produce the H₂O₂. The Au@Pt nanoflowers of the sensor can catalyze the decomposition of H₂O₂, which causes electron transfer to produce current change. Therefore, the content of IMP is indirectly obtained by monitoring the current change. The results showed that the linear range of the double-enzyme biosensor was 0.04~17 g L⁻¹, the correlation coefficient was 0.9964, and the detection limit was 2.73 ng mL⁻¹. The biosensor had great reproducibility and stability. Compared with high-performance liquid chromatography, biosensors could quickly and accurately detect the content of inosine monophosphate in meat, and provided a better method to detect the quality index of meat products.

中文翻译：

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使用双酶传感器检测肉中的肌苷一磷酸（IMP）

摘要

肌苷一磷酸（IMP）被认为是肉类风味的重要组成部分，也是评估肉类产品质量的重要指标。本文开发了一种新型的双酶生物传感器，用于肌苷酸的定量检测以评估肉的质量。利用MXene材料的电导率和Au @ Pt纳米花催化H ₂ O ₂氧化的能力，组装并制备了双酶生物传感器，用于灵敏快速检测IMP。MXene-Ti ₃ C ₂Tx材料具有类似于石墨烯的二维纳米结构，并且具有金属导电性和良好的生物相容性。MXene被用作5'-核苷酸酶和黄嘌呤氧化酶的载体，具有良好的生物环境和稳定的微环境。具有核-壳结构的双金属纳米花比单金属具有更好的催化H ₂ O _2的能力。双酶水解IMP产生H ₂ O ₂。传感器的Au @ Pt纳米花可以催化H ₂ O ₂的分解，这会导致电子转移产生电流变化。因此，通过监视电流变化间接获得IMP的含量。结果表明，双酶生物传感器的线性范围为0.04〜17 g L ^-1，相关系数为0.9964，检出限为2.73 ng mL ^-1。该生物传感器具有很高的重现性和稳定性。与高效液相色谱法相比，生物传感器能够快速，准确地检测肉中肌苷一磷酸的含量，为检测肉类产品的质量指标提供了更好的方法。