Abstract

This work was carried out to develop a high-performance liquid chromatography tandem mass spectrometry method for simultaneous determination of 11 marker compounds in phytoestrogen-rich food and dietary supplements (raw materials and formulated products) prepared from various sources: soy, red clover, chastetree, hop, and flax. The method was fully validated and showed detection limits varying from 0.2 to 1.5 μg/mL, average recoveries ranging from 95 to 105%, and relative standard deviation values lower than 4.98%. Evidently lower amounts of phytoestrogens were found in food samples than in the dietary supplements. Calculations for the actual daily dose of total phytoestrogens ranged from 0.01 to 6.61 mg among the different products. All derived total phytoestrogen amounts turned out to be below the total content stated by manufacturers (less than 63% of the claimed specifications). Moreover, two samples did not contained phytoestrogens at all. The most eye-catching batch-to-batch deviations were represented by chasteberry-based products (relative standard deviation higher than 9.8%).

中文翻译：

---

液相色谱-串联质谱法对食品，原料和膳食补充剂中的植物雌激素进行多目标筛选

摘要

开展这项工作的目的是开发一种高效液相色谱串联质谱法，该方法可同时测定由多种来源制得的富含植物雌激素的食品和膳食补充剂（原料和配方产品）中的11种标记物：大豆，红三叶草，贞洁树，啤酒花和亚麻。该方法已经过充分验证，检测限从0.2到1.5μg/ mL不等，平均回收率在95到105％之间，相对标准偏差值低于4.98％。与食物补充剂相比，食物样品中的植物雌激素含量明显较低。在不同产品中，总植物雌激素的实际日剂量计算范围为0.01至6.61 mg。结果证明，所有衍生的总植物雌激素含量均低于制造商规定的总含量（少于要求保护的规格的63％）。而且，两个样品根本不含植物雌激素。最为引人注目的批次间偏差由基于Chasteberry的产品代表（相对标准偏差高于9.8％）。