Abstract

Flumequine (FLU) is widely used as an antibiotic in the treatment and control of animal diseases, which may result in the presence of residues harmful to humans in food products of animal origin. A novel and fast biomimetic enzyme-linked immunosorbent assay (BELISA) was developed for the determination of FLU based on a molecularly imprinted polymer (MIP) as a selective affinity agent. With an optimized molar ratio of FLU to methacrylic acid (MAA) of 1:2 determined by molecular dynamic simulations, the MIP was directly synthesized on the surface of a 96-well plate (as solid support) with FLU as the template molecule, MAA as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, and azodiisobutyronitrile (AIBN) as the initiator. Under the optimized conditions, the BELISA method was established with a sensitivity (IC₅₀) of 0.141 μg/mL and a detection limit (IC₁₅) of 1.09 × 10⁻³ μg/mL. The developed method was applied to determine FLU concentrations in spiked beef and shrimp samples, with satisfactory recovery values ranging from 80.7 to 92.2% with relative standard deviations (n = 3) between 3.3% and 7.0%. This method can be used for the rapid determination of FLU in animal foods and has promising applications in food safety monitoring.

中文翻译：

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仿生酶联免疫吸附法快速检测动物性食品中氟胺的研究

摘要

Flumequine（FLU）被广泛用作动物疾病的治疗和控制中的抗生素，这可能导致动物源食品中存在对人类有害的残留物。基于分子印迹聚合物（MIP）作为选择性亲和剂，开发了一种新型的快速仿生酶联免疫吸附测定（BELISA）用于测定FLU。通过分子动力学模拟确定FLU与甲基丙烯酸（MAA）的最佳摩尔比为1：2，以FLU为模板分子，将MIP直接合成在96孔板（作为固相支持物）的表面上作为功​​能单体，乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂，偶氮二异丁腈（AIBN）作为引发剂。在优化的条件下，建立了具有灵敏度的BELISA方法（IC₅₀）0.141μg/ mL和，检测下限（IC的₁₅的1.09×10）^-3 μg/ mL的。所开发的方法用于测定加标牛肉和虾样品中的FLU浓度，回收率令人满意，范围为80.7至92.2％，相对标准偏差（n = 3）在3.3％至7.0％之间。该方法可用于动物食品中FLU的快速测定，在食品安全监测中具有广阔的应用前景。