In our work, we developed the synthesis of new polyfunctional pegylated trehalose derivatives and evaluated their cryoprotective effect using flow cytometry. We showed that new compounds (modified trehaloses) bound to appropriate extracellular polymeric cryoprotectants could be helpful as a chemical tool for the evaluation of their potential toxic cell membrane influences. Our aim was to form a chemical tool for the evaluation of cryoprotectant cell membrane influences, which are still not easily predicted during the freezing/thawing process. We combined two basic cryoprotectants: polyethyleneglycols (PEGs) and trehalose in the new chemical compounds—pegylated trehalose hybrids. If PEG and trehalose are chemically bound and trehalose is adsorbed on the cell surface PEGs molecules which are, due to the chemical bonding with trehalose, close to the cell surface, can remove the cell surface hydration layer which destabilizes the cell membrane. This was confirmed by the comparison of new material, PEG, trehalose, and their mixture cryoprotective capabilities.

中文翻译：

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新聚乙二醇化海藻糖衍生物的简单合成作为潜在评估对细胞膜冷冻保护作用的化学工具

在我们的工作中，我们开发了新型多功能聚乙二醇化海藻糖衍生物的合成，并使用流式细胞术评估了它们的冷冻保护作用。我们表明，与适当的细胞外聚合物冷冻保护剂结合的新化合物（改性海藻糖）可能有助于作为评估其潜在毒性细胞膜影响的化学工具。我们的目标是形成一种用于评估冷冻保护剂细胞膜影响的化学工具，这些影响在冷冻/解冻过程中仍然不容易预测。我们在新化合物中结合了两种基本的冷冻保护剂：聚乙二醇 (PEG) 和海藻糖——聚乙二醇化海藻糖杂化物。如果 PEG 和海藻糖化学结合，并且海藻糖吸附在细胞表面 PEGs 分子上，由于与海藻糖的化学键合，靠近细胞表面，可以去除使细胞膜不稳定的细胞表面水化层。这通过新材料、PEG、海藻糖及其混合物的冷冻保护能力的比较得到证实。