INTRODUCTION Vitis labrusca L. grapes are largely cultivated in Brazil, but the tropical climate negatively affects the phenols content, especially anthocyanin. According to the projections of the incoming climatic changes, the climate of several viticulture zone might change to tropical. Therefore, researches are focusing on increasing grape phenols content; with methyl jasmonate application (MeJa) is considered a good alternative. OBJECTIVES The aim was to investigate with an untargeted approach the metabolic changes caused by the MeJa pre-harvest application on two Vitis labrusca L. cultivars grapes, both of them grown in two Brazilian regions. METHODS Isabel Precoce and Concord grapes cultivated under subtropical climate, in the south and southeast of Brazil, received MeJa pre-harvest treatment. Grape metabolome was extracted and analyzed with a MS based metabolomics protocol by UPLC-HRMS-QTOF. RESULTS Unsupervised data analysis revealed a clear separation between the two regions and the two cultivars, while supervised data analysis revealed biomarkers between the MeJa treatment group and the control group. Among the metabolites positively affected by MeJa were (a) flavonoids with a high degree of methylation at the B-ring (malvidin and peonidin derivatives and isorhamentin) for Isabel Precoce grapes; (b) glucosides of hydroxycinnamates, gallocatechin, epigallocatechin and cis-piceid for Concord grapes; and (c) hydroxycinnamates esters with tartaric acid, and procyanidins for the Southeast region grapes. CONCLUSION These results suggest that MeJa can be used as elicitor to secondary metabolism in grapes grown even under subtropical climate, affecting phenolic biosynthesis.

中文翻译：

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LC-MS非靶向方法表明，茉莉酸甲酯在巴西葡萄亚种上的应用增加了酚类物质的含量。

引言葡萄在巴西主要种植，但是热带气候对酚含量，尤其是花色苷有负面影响。根据即将到来的气候变化的预测，一些葡萄栽培区的气候可能会变为热带。因此，研究集中在增加葡萄中酚的含量上。茉莉酸甲酯的应用（MeJa）被认为是一个很好的选择。目的目的是通过无针对性的方法研究MeJa收获前对两种葡萄（Vitis labrusca L. cultivars）葡萄的MeJa收获前施用引起的代谢变化，这两种葡萄均生长在两个巴西地区。方法在巴西南部和东南部，在亚热带气候下种植的伊莎贝尔·普瑞科斯和康科德葡萄接受了MeJa收获前处理。通过UPLC-HRMS-QTOF提取基于MS的代谢组学方案对葡萄代谢组进行分析。结果无监督数据分析显示两个区域和两个品种之间有明显的分离，而监督数据分析显示了MeJa治疗组和对照组之间的生物标记。受MeJa正面影响的代谢物包括（a）伊莎贝尔（Isabel Precoce）葡萄在B环上具有高度甲基化的黄酮类化合物（malvidin，peonidin衍生物和isorhamentin）；（b）康科德葡萄的羟基肉桂酸酯，没食子儿茶素，表没食子儿茶素和顺式-哌啶糖苷；（c）与酒石酸的羟基肉桂酸酯和原花青素用于东南地区的葡萄。