OBJECTIVE To explore the inhibitory effects of zoledronate (ZOL) on adipose-derived stem cells (ADSCs) into osteoblasts for repairing jaw necrosis. METHODS ADSCs were induced to differentiate into osteoblasts. The differentiation characteristics of osteoblasts was observed under inverted microscope by alizarin red staining. The transwell assay was performed to evaluate the migration of ADSCs co-cultured with osteoblasts and divided into ZOL group treated with ZOL and N-ZOL group without ZOL treatment. The differentiation and proliferation characteristics of ADSCs differentiated osteoblasts were observed respectively. The expression of CTSK (Cathepsin K) and FGFR3 (Fibroblast growth factor receptor 3) in osteoblasts were analyzed by immunofluorescence and western blot. RESULTS The differentiation degree and proliferation of ADSCs to osteoblasts in N-ZOL group were both higher than those in ZOL group. The migratory cell number in ADSCs differentiation in ZOL group was higher than that of N-ZOL group. The protein expression of CTSK and FGFR3 in ADSCs differentiated to osteoblasts in ZOL group was higher than that in N-ZOL group. CONCLUSION The differentiation of ADSCs into osteoblasts is significantly inhibited by ZOL. Due to this reason, it may be difficult to achieve good results by ZOL induced ADSCs into osteoblasts in repairing jaw necrosis.

中文翻译：

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唑来膦酸盐在修复颌骨坏死时抑制了脂肪干细胞向成骨细胞的分化潜能。

目的探讨唑来膦酸（ZOL）对成骨细胞脂肪干细胞（ADSCs）修复颌骨坏死的抑制作用。方法诱导ADSCs分化为成骨细胞。倒置显微镜下茜素红染色观察成骨细胞的分化特征。进行transwell分析以评估与成骨细胞共培养的ADSCs的迁移，将其分为用ZOL处理的ZOL组和未经ZOL处理的N-ZOL组。分别观察了ADSCs分化成骨细胞的分化和增殖特性。用免疫荧光和蛋白质印迹法分析成骨细胞中CTSK（组织蛋白酶K）和FGFR3（成纤维细胞生长因子受体3）的表达。结果N-ZOL组ADSCs向成骨细胞的分化程度和增殖均高于ZOL组。ZOL组ADSCs分化的迁移细胞数高于N-ZOL组。ZOL组分化为成骨细胞的ADSCs中CTSK和FGFR3的蛋白表达高于N-ZOL组。结论ZOL可显着抑制ADSCs向成骨细胞的分化。由于这个原因，在修复颌骨坏死中，ZOL诱导的ADSCs进入成骨细胞可能难以获得良好的效果。ZOL组分化为成骨细胞的ADSCs中CTSK和FGFR3的蛋白表达高于N-ZOL组。结论ZOL可显着抑制ADSCs向成骨细胞的分化。由于这个原因，在修复颌骨坏死中，ZOL诱导的ADSCs进入成骨细胞可能难以获得良好的效果。ZOL组分化为成骨细胞的ADSCs中CTSK和FGFR3的蛋白表达高于N-ZOL组。结论ZOL可显着抑制ADSCs向成骨细胞的分化。由于这个原因，在修复颌骨坏死中，ZOL诱导的ADSCs进入成骨细胞可能难以获得良好的效果。