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Integrating Exposure Knowledge and Serum Suspect Screening as a New Approach to Biomonitoring: An Application in Firefighters and Office Workers.
Environmental Science & Technology ( IF 11.4 ) Pub Date : 2020-03-15 , DOI: 10.1021/acs.est.9b04579
Rachel Grashow 1 , Vincent Bessonneau 1 , Roy R Gerona 2 , Aolin Wang 3 , Jessica Trowbridge 4 , Thomas Lin 2 , Heather Buren 5 , Ruthann A Rudel 1 , Rachel Morello-Frosch 4, 6
Affiliation  

Firefighters (FF) are exposed to recognized and probable carcinogens, yet there are few studies of chemical exposures and associated health concerns in women FFs, such as breast cancer. Biomonitoring often requires a priori selection of compounds to be measured, and so, it may not detect relevant, lesser known, exposures. The Women FFs Biomonitoring Collaborative (WFBC) created a biological sample archive and conducted a general suspect screen (GSS) to address this data gap. Using liquid chromatography-quadrupole time-of-flight tandem mass spectrometry, we sought to identify candidate chemicals of interest in serum samples from 83 women FFs and 79 women office workers (OW) in San Francisco. We identified chemical peaks by matching accurate mass from serum samples against a custom chemical database of 722 slightly polar phenolic and acidic compounds, including many of relevance to firefighting or breast cancer etiology. We then selected tentatively identified chemicals for confirmation based on the following criteria: (1) detection frequency or peak area differences between OW and FF; (2) evidence of mammary carcinogenicity, estrogenicity, or genotoxicity; and (3) not currently measured in large biomonitoring studies. We detected 620 chemicals that matched 300 molecular formulas in the WFBC database, including phthalate metabolites, phosphate flame-retardant metabolites, phenols, pesticides, nitro and nitroso compounds, and per- and polyfluoroalkyl substances. Of the 20 suspect chemicals selected for validation, 8 were confirmed-including two alkylphenols, ethyl paraben, BPF, PFOSAA, benzophenone-3, benzyl p-hydroxybenzoate, and triphenyl phosphate-by running a matrix spike of the reference standards and using m/z, retention time, and the confirmation of at least two fragment ions as criteria for matching. GSS provides a powerful high-throughput approach to identify and prioritize novel chemicals for biomonitoring and health studies.

中文翻译:

将接触知识和血清可疑检查相结合作为一种生物监测新方法:在消防员和办公室工作人员中的应用。

消防员(FF)暴露于公认的和可能的致癌物中,但是很少有研究对女性FFs中的化学暴露及相关健康问题进行研究,例如乳腺癌。生物监测通常需要事先选择要测量的化合物,因此,它可能无法检测到相关的,鲜为人知的暴露。妇女FFs生物监测合作组织(WFBC)创建了一个生物样本档案库,并进行了一般嫌疑筛查(GSS)以解决这一数据缺口。我们使用液相色谱-四极杆飞行时间串联质谱,从旧金山的83名女性FF和79名女性上班族(OW)的血清样品中鉴定出感兴趣的候选化学物质。我们通过将血清样品中的准确质量与722种弱极性酚和酸性化合物的定制化学数据库进行匹配(包括许多与消防或乳腺癌病因有关的信息),来确定化学峰。然后,我们根据以下标准选择经过初步鉴定的化学品进行确认:(1)OW和FF之间的检测频率或峰面积差异;(2)乳癌致癌性,雌激素性或遗传毒性的证据;(3)目前尚未在大型生物监测研究中进行衡量。我们在WFBC数据库中检测到620种与300个分子式匹配的化学物质,包括邻苯二甲酸酯代谢物,磷酸盐阻燃代谢物,酚,农药,硝基和亚硝基化合物以及全氟和多氟烷基物质。在选择用于验证的20种可疑化学品中,通过运行参考标准品的基质加标峰并使用m / z,保留时间和确证性,确认了8种化合物,包括两种烷基酚,对羟基苯甲酸乙酯,BPF,PFOSAA,二苯甲酮3,对羟基苯甲酸苄酯和磷酸三苯酯。至少两个碎片离子作为匹配标准。GSS提供了一种功能强大的高通量方法,可用于生物监控和健康研究的新型化学物质的识别和优先排序。
更新日期:2020-03-16
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