The yellow margin (ym) gene was mapped to a 30-kb genomic region in potato and the mutation of a pectate lyase gene led to this phenotype. The practice of clonally propagating potato (Solanum tuberosum L.), which has been lasted for thousands of years, has caused the accumulation of deleterious alleles. Despite yellow margin (ym) being a common cause of a detrimental weak-vigor phenotype and reduced yield in diploid potato, the underlying gene has eluded discovery to date. In this paper, we mapped the ym gene to a 30-kb region containing four annotated genes. Among them, PGSC0003DMG402023481 encodes a pectate lyase-like gene (StPLL) with lower expression in ym plants than in the wild-type plants. PCR amplification confirmed a 4.1-kb deletion in the mutant allele of StPLL. Knockout of StPLL in diploid potato resulted in a similar phenotype with the ym plants. This study not only characterizes the ym allele but also provides the molecular tools to select and purge it from populations, while also deepening our understanding of the morphogenesis in potato.

中文翻译：

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PECTATE LYASE-LIKE 基因的突变是造成马铃薯黄缘表型的原因。

黄色边缘 (ym) 基因被定位到马铃薯的一个 30-kb 基因组区域，果胶酸裂解酶基因的突变导致了这种表型。无性繁殖马铃薯（Solanum tuberosum L.）的做法已经延续了数千年，导致有害等位基因的积累。尽管黄缘 (ym) 是二倍体马铃薯有害的弱活力表型和减产的常见原因，但其潜在基因迄今仍未被发现。在本文中，我们将 ym 基因映射到包含四个注释基因的 30 kb 区域。其中，PGSC0003DMG402023481编码一种果胶酸裂解酶样基因（StPLL），在ym植物中的表达低于野生型植物。PCR 扩增证实 StPLL 的突变等位基因有 4.1-kb 缺失。在二倍体马铃薯中敲除 StPLL 导致与 ym 植物相似的表型。这项研究不仅表征了 ym 等位基因，还提供了从种群中选择和清除它的分子工具，同时也加深了我们对马铃薯形态发生的理解。